Earticle

초록

영어

The object of this study was to investigate the immune-enhancing effects of Chlorella vulgaris (CV) on a deteriorated immune function by a protein-energy malnutrition (PEM) diet. Unicellular algae, CV were used as a biological response modifier. Male C57BL/6J mice were fed for 15 days with standard diet or a PEM diet, which is associated with decreased host immune defense. After 8 days, mice in the PEM diet group were orally administered by 0.05, 0.1, and 0.15 g/kg body weight of CV or distilled water. Nutritional parameters, and interferon (IFN)-γ levels were significantly increased in the blood serum of the CV (0.15 g/kg)-treated group (29.6±2.8 pg/mL) compared to the non-treated PEM group (4.1±0.4 pg/mL, p<0.05). In addition, cell proliferation and production of cytokines were investigated via a CV (0.01, 0.1, and 1 mg/mL)
treatment using a human T cell line MOLT-4 cell. The CV treatment (1 mg/mL) significantly increased the production of both IFN-γ and interleukin (IL)-2 (51.3±3.4 and 285.9±18.8 pg/mL, respectively) compared to the control (51.3±3.4 and 442.6±14.3 pg/mL, respectively), but did not affect the production of IL-4. These results suggest that CV may be useful in improving the immune function.

목차

Abstract
 Introduction
 Materials and Methods
  Animals
  Treatment of CV
  Preparation and ingredient analysis of blood serum
  Counting mononuclear cell suspensions from spleen,superficial and mesenteric lymph nodes
  MOLT-4 cell cultures
  MTT assay
  Cytokine assay
  Statistical analysis
 Results and Discussion
  Effect of CV on blood nutritional parameters
  Effect of CV on mononuclear cell numbers in thespleen and the superficial and mesenteric lymph nodes
  Effect of CV on the production of IFN-γ in blood serum
  Effect of CV on human T cell line MOLT-4 cellproliferation
  Effect of CV on the production of IFN-γ, IL-2, and IL-4 in human T cell line MOLT-4 cells
 References

키워드

저자

• Hyo-Jin An [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• Hong-Kun Rim [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University, ]
• Jong-Hyun Lee [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• Jin-Woo Hong [ Department of Oriental Internal Medicine, Pusan National University ]
• Na-Hyung Kim [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• Noh-Yil Myung [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• Phil-Dong Moon [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• In-Young Choi [ Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University ]
• Ho-Jeong Na [ Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University ]
• Hyun-Ja Jeong [ Biochip Research Center, Hoseo University ]
• Hyeung-Suk Park [ Daesang Corp., WellLife R&D Center ]
• Jae-Gab Han [ Daesang Corp., WellLife R&D Center ]
• Jae-Young Um [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ]
• Hyung-Min Kim [ College of Oriental Medicine, Institute of Oriental Medicine, Oriental Medical Science Center, Kyung Hee University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664