Momordica charantia Protects against Cytokine-induced Apoptosis in Pancreatic β-Cells
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- 발행기관
- 한국식품과학회 바로가기
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- 간행물
- Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
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- 통권
- Volume 17 Number 5 (2008.10)바로가기
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- 페이지
- pp.947-952
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A88415
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The unripe fruit of Momordica charantia (MC) has been shown to possess antidiabetic activity. However, the mechanism of its antidiabetic action has not been fully understood. In this study, the effects of the aqueous ethanolic extract of MC (AEE-MC) were evaluated on the apoptosis in pancreatic β-cells treated with a combination of the cytokines, interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ. In MIN6N8 cells, the inhibitory effect of AEE-MC was
significantly observed at 2 to 50 μg/mL: a 26.2 to 55.6% decrease of cytoplasmic DNA fragments quantified by an immunoassay.
The molecular mechanisms, by which AEE-MC inhibited β-cell apoptosis, appeared to involve the inhibition on the expression of p21, Bax, and Bad, the up-regulation of Bcl-2 and Bcl-XL, and the inhibition on the cleavage of caspase-9, -7, and -3 and poly (ADP-ribose) polymerase. This study suggests that MC may inhibit cytokine-induced apoptosis in β-cells and, thus, may contribute via this action to the antidiabetic influence in diabetes.
목차
Abstract
Introduction
Materials and Methods
Momordica charantia (MC)
Preparation of MC extract
Cell lines and reagents
Exposure to proinflammatory cytokines
Cell survival assay
Measurement of cell death
Preparation of cell lysates and immunoblotting
Statistical analysis
Results and Discussion
Effects of cytokines on cell viability, apoptosis, and proapoptoticproteins in MIN6N8 cells
Effect of MC extracts on cell viability, cytokine-inducedcytotoxicity, and apoptosis in MIN6N8 cells
Effects of AEE-MC on cytokine-mediated activation ofPARP and caspases
Effects of AEE-MC on the expressions of Bcl-2 familyand p21 in cytokine-treated MIN6N8 cells
References
키워드
Momordica charantia cytokine apoptosis MIN6N8 cell diabetes Bcl-2 family저자
간행물 정보
발행기관
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- 발행기관명
- 한국식품과학회 [Korean Society of Food Science and Technology]
- 설립연도
- 1970
- 분야
- 농수해양>식품과학
- 소개
- 본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.
간행물
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- 간행물명
- Food Science and Biotechnology
- 간기
- 격월간
- pISSN
- 1226-7708
- 수록기간
- 1992~2009
- 십진분류
- KDC 574 DDC 664
이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 5
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