Earticle

초록

영어

Covalent cross-links between a number of proteins and peptides explain why transglutaminase may be widely used by food processing industries. The objective of this work was optimization of the fermentation process to produce transglutaminase from a new microbial source, the Streptomyces sp. P20. The strategy adopted to modify the usual literature media was: (1) fractional factorial design (FFD) to elucidate the key medium ingredients, (2) central composite design (CCD) to optimise the concentration of the key components. Optimization of the medium resulted in not only an 86% increase in microbial transglutaminase activity as compared to the media cited in the literature, but also a reduction in the production cost. Optimal fermentation conditions - namely temperature and agitation rate - were also studied, using CCD methodology. Usual conditions of 30°C and 100 rpm were within the optimal area. All other parameters for enzyme production were experimentally proven to be optimum fermentation conditions.

목차

Abstract
 Introduction
 Materials and Methods
  Materials
  Selective isolation and preservation of actinomycetes
  Microorganisms selection for MTGase production
  Preliminary tests Production of MTGase using a highviscosity medium:
  Selection of the carbon and nitrogen sources for MTGaseproduction:
  Optimization procedure for the production parametersand experimental design
  Elucidation of the significant components using a FFD:
  Optimization of key ingredient concentrations using aCCD:
  Temperature and agitation optimization procedure andexperimental design:
  Influence of inoculum size
  Kinetic study
 Results and Discussion
  Microorganisms selection for MTGase production
  Preliminary tests Production of MTGase using a highviscosity medium:
  Selection of the carbon and nitrogen sources for MTGaseproduction:
  Elucidation of the significant components using a FFD:
  Optimization of key ingredient concentrations using aCCD:
  Temperature and agitation optimization procedure andexperimental design:
  Influence of inoculum size and kinetic study
 References

키워드

저자

• Juliana Alves Macedo [ Brazilian Collection of Environmental and Industrial Microorganisms (CBMAI), Microbial Resource Division ] Corresponding author
• Lara Durães Sette [ Brazilian Collection of Environmental and Industrial Microorganisms (CBMAI), Microbial Resource Division, ]
• Hélia Harumi Sato [ Food Science Department, Faculty of Food Engineering, Universidade Estadual De Campinas (UNICAMP) ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664