The mobility of water in set yogurt during fermentation was studied using nuclear magnetic resonance (NMR) relaxometry. The spin-spin relaxation was analyzed using a 2-fraction model, resulting in 2 spin-spin relaxation time constants T21 and T22. Both T21 and T22 exhibited rapid changes between 2 and 4 hr of fermentation, coinciding with the drop in pH and the rise in lactic acid bacteria count. The spin-lattice relaxation time T1 increased over the fermentation period. Both T1 and T2 showed an increase in the mobility of water upon gel formation during fermentation. Water redistribution within the gel matrix due to casein aggregation and structure forming may be responsible for the changes in mobility.
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Abstract Introduction Materials and Methods Materials NMR analysis pH assay Lactic acid bacteria enumeration Results and Discussion Changes in pH and lactic acid bacteria during fermentation NMR relaxometry Changes in water mobility during fermentation References