Earticle

초록

영어

In order to enhance the efficacy of norovirus detection by reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR, this study developed a norovirus mRNA concentration method using poly oligo dT-conjugated magnetic beads. An efficient norovirus detection protocol was performed on commercial ham using 2 viral elution buffers (glycine buffer and Tris beef extract buffer) and 2 concentration solutions [polyethylene glycol (PEG) and zirconium hydroxide]. The different approaches were verified by RT-PCR and nested PCR. This method was performed on ham in less than 8 hr by artificial inoculation of serial dilutions of the virus ranging from 1,000 to 1 RT-PCR unit/mL. The viral extraction and concentration method had 10-fold higher sensitivity using the combination of Tris beef extract buffer and PEG as compared to glycine buffer and zirconium hydroxide. This method proved that RT-PCR and nested PCR have the sensitive ability to detect norovirus in commercial ham, in that norovirus was successfully detected in artificially contaminated samples at a detection level as low as 1-10 RT-PCR unit/mL. Overall, such a detection limit suggests this protocol is both quick and efficient in terms of its potential use for detecting norovirus in meat products.

목차

Abstract 
 Introduction
 Materials and Methods 
  Virus
  Virus extraction and concentration
  Extraction of viral RNA
  Primers and PCR
 Results and Discussion
  RNA purification and optimal PCR conditions
  Concentration of norovirus
 References

키워드

저자

• Seok Ryel Kim [ Division of Food Science and Aqualife Medicine, Chonnaam National University ]
• Duwoon Kim [ Division of Food Science and Aqualife Medicine, Chonnaam National University ]
• Ki-Sung Kwon [ Korea Food & Drug Administration ]
• In-Gyun Hwang [ Korea Food & Drug Administration ]
• Myung-Joo Oh [ Division of Food Science and Aqualife Medicine, Chonnaam National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664