Kyo-Young Seo, Sun-Kyung Heo, Dong-Ho Bae, Dong-Hwan Oh, Sang-Do Ha
언어
영어(ENG)
URL
https://www.earticle.net/Article/A87814
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원문정보
초록
영어
A mathematical model was developed for predicting the growth rate of Enterobacter sakazakii in tryptic soy broth medium as a function of the combined effects of temperature (5, 10, 20, 30, and 40℃), pH (4, 5, 6, 7, 8, 9, and 10), and the NaCl concentration (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10%). With all experimental variables, the primary models showed a good fit (R²=0.8965 to 0.9994) to a modified Gompertz equation to obtain growth rates. The secondary model was 'In specific growth rate=-0.38116+(0.01281*Temp)+(0.07993*pH)+(0.00618*NaCl)+(-0.00018*Temp²)+(-0.00551*pH²)+(-0.00093*NaCl²)+(0.00013*Temp*pH)+(-0.00038*Temp*NaCl)+(-0.00023*pH*NaCl)'. This model is thought to be appropriate for predicting growth rates on the basis of a correlation coefficient (r) 0.9579, a coefficient of determination (R²) 0.91, a mean square error 0.026, a bias factor 1.03, and an accuracy factor 1.13. Our secondary model provided reliable predictions of growth rates for E. sakazakii in broth with the combined effects of temperature, NaCl concentration, and pH.
목차
Abstract Introduction Materials and Methods Experimental design Preparation and inculation of culture media Growth temperature and growth rate measurement Primary modeling Secondary modeling Evaluation of model performance Results and Discussion Primary References