Earticle

초록

영어

Acanthopanax divaricatus var. albeofructus (ADA) have been shown to have various levels of activity such as antioxidant, anticancer, antivirus, and immunostimulatory effects. However, little is known about its mechanism related to the modulation of immune activities. In this study, a water extract of ADA leaves were used to treat human peripheral blood mononuclear cells (hPBMC) to determine the underlying mechanisms for the immunostimulatory effects. To characterize its immunomodulatory activity, the secretion level of various cytokines including IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ, and TNF-α were measured using enzyme-linked immunosorbent assay (ELISA). Treatment of hPBMC with ADA leaf extract in an in vitro experiment induced various Th1 cytokines in a dose-dependent manner. A significant increase of IL-2, IL-12, IFN-γ, and TNF-α secretion was observed in the presence of ADA leaf extract. In contrast, Th2 cytokines including IL-4 and IL-6 were suppressed. There was no significant change in IL-10 release. Our results showed an increase in Th1 and a decrease in Th2 cytokine secretion which suggests that ADA may influence the immune response towards a predominance of Th1 cytokines in the immune system.

목차

Abstract
 Introduction
 Materials and Methods
  Preparation of Acanthopanax divaricatus var. albeofructus leaf extract 
  Cytotoxicity assay
  Cytokine secretion
  Statistical analysis
 Results and Discussion
  Cytotoxicity of ADA leaf extract
  Effect of ADA leaf extract on Th1 cytokine cytokine secretion
  Effect of ADA leaf extract on Th2 cytokine secretion
 References

키워드

저자

• Su-Yun Lyu [ Department of Herbal Medicinal Pharmacology, Daegu Haamy University ]
• Won-Bong Park [ College of Natural Sciences, Seoul Women's University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664