Won-Bo Shim, Jung-Sook Kim, Ji-Young Kim, Jin-Gil choi, Jung-Hyun Je, Nima Sergevena Kuzmina, Sergei Alexandrovich Eremin, Duck-Hwa Chung
언어
영어(ENG)
URL
https://www.earticle.net/Article/A87811
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.
목차
Abstract Introduction Materials and Methods Materials and chemicals Preparation of immunoreagents DC-ELISA ICG strip-test Sample preparation Analysis of AFB1 in artificially spiked and natural samples Results and Discussion Characterization of DC-ELISA Characterization of ICG strip-test Assessment of the matrix effect from grain and feed samples Analytical comparison of DC-ELISA and ICG striptest Monitoring of AFB1 in natural samples References