Earticle

초록

영어

Cancer chemopreventive effects can be exerted through the induction of phase II detoxification enzymes and the inhibition of inflammatory responses. In this study, the cancer chemopreventive effects and anti-inflammatory responses of 30 seaweed extracts were examined. The extracts of Dictyota coriacea and Cutleria cylindrica exhibited the high chemoprevention index, having 4.36 and 4.66, respectively. They also activated antioxidant response element at 100㎍/mL by about 3-fold while did not activate xenobiotic response element. Seven seaweed extracts, Ishige okamurae, Desmarestia ligulata, Desmarestia viridis, Dictyopteris divaricata, D. coriacea, Sargassum horneri, and Sargassum yezoense, showed significant inhibition on nitric oxide (NO) and prostaglandin E2 (PGE2) production in a dose-dependant manner in 5-20㎍/mL. These seaweed extracts could be used as food materials for cancer chemoprevention. D. coriacea could contain potential chemopreventive agents not only that regulate genes via an ARE-dependent mechanism but also prevent the inflammation through inhibition of NO and PGE2 production.

목차

Abstract 
 Introduction
 Materials and Methods 
  Seaweed materials
  Preparation of 30 seaweed extracts
  Cell culture
  Cell viability
  Quinone reductase assay 
  Transient transfection and ARE/XRE activation assay using CAT-ELISA
  Measurement of nitrite production using Griess reagent
  Measurement of PGE₂production by enzyme-linked immunosorbent assay(ELISA)
 Results and Discussion
  Chemoprevention index (CI) of seaweed extracts in Hepalclc7 cells
  Activation of ARE by 4 seaweed extracts in HepG2 cells
  Inhibition of NO production in RAW264.7 cells
  Inhibition of PGE₂production in RAW264.7 cells  
 References

키워드

저자

• Saet Byoul Lee [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Joo Young Lee [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Dae-Geun Song [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Cheol-Ho Pan [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Chu Won Nho [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Min Cheol Kim [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Eun Ha Lee [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Sang Hoon Jung [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ]
• Hyung-Seop Kim [ Department of Biology, Kangnung National University ]
• Yeong Shik Kim [ College of Pharmacy Natural Products Research Institute, Seoul National University ]
• Young Hun Um [ Natural Product Research Center, Korea institute of Science and Technology Gangneung Institute ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664