Ji-Hye Ahn, Jae-Hwan Kim, Su-Youn Kim, Woo-Young Lee, Hae-Yeong Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A87802
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원문정보
초록
영어
A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.
목차
Abstract Introduction Materials and Methods Maize samples DNA extraction Oligonucleotide primers Multiplex PCR conditions Results and Discussion Specificity of primer pairs for a multiplex PCR References