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Impact of Sodium Copper Chlorophyllin on Mercury absorption Using an in Vitro digestion with Human Intestinal Cell Model

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 17 Number 3 (2008.06)바로가기
  • 페이지
    pp.564-568
  • 저자
    Han-Joon Hwang, Soon-Mi Shim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A87801

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원문정보

초록

영어
The effects of sodium copper chlorophyllin (SCC) on bioaccessibility and uptake of mercury from fish were investigated using an in vitro digestion coupled with a Caco-2 cell. Fish along with SCC was subjected to a simulated in vitro digestion, which simulates both the gastric and small intestinal phase in vivo. Mercury bioaccessibility, the amount of mercury released from fish to aqueous phase following a digestion, was measured. Various amounts of SCC (0.1-25 mg) significantly reduced mercury bioaccessibility in a dose dependent manner by 49-89% compared to the negative control (fish without SCC) (p<0.05). Mercury bioaccessibility in varying molar ratios of mercury to positive control, 2,3-dimercapto-1-propane sulfonate (DMPS) was between 24 and 52%. Mercury uptake by Caco-2 cells from test media containing aqueous phase following in vitro digestion was measured after 6 hr incubation at 37℃. Cellular mercury uptake with increasing amount of SCC ranged from 0.352 to 0.052㎍ mercury/mg protein, while those in DMPS treatment were between 0.14 and 0.27㎍ mercury/mg protein. Our study suggests that SCC can reduce mercury absorption following fish consumption and may be efficient as a synthetic chelating agent for long term chronic mercury exposure in fish eating populations.

목차

Abstract
 Introduction
 Materials and Methods
  Materials
  Test meals preparation
  In vitro digestion
  Uptake of mercury by Caco-2 intestinal cells
  Assessment of toxicity and cellular viability
  Protein assay
  Determination of mercury
  Statistical analysis
 Results and Discussion
  Mercury bioaccessility
  Mercury uptake by Caco-2 cell
  Effect of SCC on mercury bioaccessibility
  Effects of SCC on mercury uptake by Caco-2 cells 
 References

저자

  • Han-Joon Hwang [ Department of Food and Biotechnology, Korea University ]
  • Soon-Mi Shim [ Research Institute of Human Ecology, Seoul National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 3

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