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Isolation and Identification of Bacillus sp. with High Protease and Amylase Activity from Sunchang Traditional Kochujang
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- 발행기관
- 한국식품과학회 바로가기
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- 간행물
- Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
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- 통권
- Volume 17 Number 3 (2008.06)바로가기
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- 페이지
- pp.519-526
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A87794
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- To improve the quality of traditional kochujang, strains with high protease and amylase activity were isolated and identified from Sunchang traditional kochujang. Twenty-three strains strongly producing protease and 16 strains strongly producing α- and β-amylase were isolated by using 1% isolated soy protein agar medium and 2% starch agar medium, respectively. Protease activities of the IA7, I5, and IA2 strain were 22.5, 21.2, and 20.6 unit/mL, respectively, and were higher than those of the other strains. Stains with high α-amylase activity included K9 (967.8 unit/mL), K14 (828.3 unit/mL), K13 (662.5 unit/mL), K8 (601.5 unit/mL), and K11 (405.9 unit/mL). The β-amylase activity of the K11 strain was the highest, 34.3 unit/mL, among the isolated strains. Based on morphological, physiological properties, and API 50CHB-kit test for assimilation of 49 carbohydrates, 8 strains selected according to protease, α-amylase, and β-amylase activities were tentatively identified as Bacillus megaterium (IA2), Bacillus subtilis (IA7, 15), Bacillus amyloliquefaciens (K8, K9, K11, and K13), and Bacillus stearothermophillus (K14). The IA7, 15, and K11 strains were finally identified as B. subtilis (99% ID) based on 16S rDNA sequencing.
목차
Abstract
Introduction
Materials and Methods
Materials
Screening of the strains with high protease activity
Measurement of protease activity
Screening of the strains with high amylase activity
Measurement of amylase activity
Identification of isolated strains
Results and Discussion
Screening and isolation of strains with high protease activity
Screening and isolation of the strains with high amylase
Morphological, physiological, and biochemical properties of the isolated stramins
Identification by 16S rDNA sequencing
References
저자
간행물 정보
발행기관
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- 발행기관명
- 한국식품과학회 [Korean Society of Food Science and Technology]
- 설립연도
- 1970
- 분야
- 농수해양>식품과학
- 소개
- 본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.
간행물
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- 간행물명
- Food Science and Biotechnology
- 간기
- 격월간
- pISSN
- 1226-7708
- 수록기간
- 1992~2009
- 십진분류
- KDC 574 DDC 664
이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 3
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