Earticle

초록

영어

After overproduction of a recombinant β-galactosidase of Bifidobacterium infantis in Pichia pastoris, a synthesis of galacto-oligosaccharides (GOS) from 36% lactose using the enzyme (170.74 U/mg) was investigated. The transgalactosylation ratio reached up to 25.2% with 83.1% conversion of initial lactose and the maximum yield of GOS was 40.6%. The GOS syrup was composed of a 13.43% galacto-oligosaccharides, 5.06% lactose, and 8.76% monosaccharides. The prebiotic effect of GOS on the growth of bifidobacteria and lactobacilli strains was investigated in vitro. The maximum growth rate of Bifidobacterium breve and Lactobacillus acidophillus in GOS syrup (5%, v/v) media were 0.49 and 0.96/hr that are higher than those in 1%(w/v) galactose and 1%(w/v) lactose containing media. However, there was no significant difference between the specific growth rates of L. acidophillus in 1%(w/v) glucose and 5%(v/v) GOS syrup. Our data showed that GOS definitely promoted the growth of B. breve ATCC 15700T and L. acidophilus ATCC 33323.

목차

Abstract
 Introduction
 Materials and Methods
  Strains and plasmid
  Media and culture conditions
  Preparation of the recombinant β-galactosidase 
  Enzyme activity and protein assay
  Analysis of GOS by high performance liquid chromatography(HPLC)
  GOS synthesis
  Growth experiments
 Results and Discussion
  Growth and enzyme production
  HPLC analysis
  GOS synthesis
  Effect of GOS on B. breve B 20S and L. acidophilus L-5
 References

키워드

저자

• Sung Je Jung [ Department of Food Science and Agricultural Chemistry, McGill University, Institute of Life Science and resources and Department of Food Science and Biotechnology, Kyung Hee University ]
• Byong Hoon Lee [ Food Research and Development Center, Agricultural And agri-Food Canada ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664