Earticle

초록

영어

Pectin was dissolved in HCl, citric acid, and deionized distilled water (DW, 2%, v/v) and irradiated at different irradiation doses (2.5-50 kGy) by gamma ray to investigate its physicochemical characteristics and biological activity. Viscosity of pectin solution was significantly decreased by irradiation up to 10 kGy, then remained constant thereafter. Gamma-irradiation increased monosaccharide and polysaccharide levels up to 30-40 kDa. Electron donating ability of pectin solution was highest when DW was added and was increased by increasing irradiation dose (p<0.05). bleaching assay revealed irradiation resulted in development of antioxidantive activity in pectin solution. Growth inhibition of cancer cell lines was observed in irradiated pectin solution in dose-dependent manner, with G36l showing the highest. Results suggested irradiation of pectin solution could be effective for preparation of functional pectin oligomer.

목차

농산가공 부산물인 펙틴을 이용하여 기능성 펙틴 올리고머를 제조할 시 감마선 조사를 이용하는 기초 자료를 마련하고자 펙틴 용액(2%, 0.05N HCl, citrate, 증류수)을 0, 2.5, 5, 10, 20, 30 및 50kGy로 감마선 조사한 후 이화학적 특성과 생리활성을 확인하였다. 펙틴 용액의 점도는 조사선량 10kGy까지 급격히 감소하였고 그 이후의 선량에서는 조사선량별 유의적인 차이가 없었다(p>0.05). 분자량은 조사선량이 증가하면서 30-40kDa의 당과 단당류가 증가하였다. 펙틴 용액의 전자공여능을 측정해본 결과 모든 선량에서 증류수 처리 펙틴 용액이 가장 높은 값을 나타내었고, 펙틴 용액 농도별 전자공여능은 모든 선량에서 유의적으로 증가하였다(p>0.05). 펙틴 용액의  bleaching assay 결과 전자공여능의 결과와 같이 조사선량이 증가할수록 높은  유지력을 나타내었으며 항산화지수(Al)도 선량이 증가함에 따라 증가하였다(p<0.05). 펙틴 용액의 5개 암세포주에 대한 생장 억제 효과는 조사 선량이 증가할수록 증가하였고 이 중 인체 유래 피부암 세포주인 G361의 생장억제 효과가 모든 처리구에서 가장 높았다. 본 실험 겉과 방사선 조사를 이용한 펙틴 용액의 저분자화를 확인할 수 있었고 생리활성이 증가하는 것으로 보아 방사선 조사가 기능성 펙틴 올리고머 제조기술에 효과적으로 사용될 수 있을 것으로 판단된다.

키워드

저자

• 강호진 [ Ho Jin Kang ]
• 조철훈 [ Cheroun Jo ]
• 권중호 [ Joong Ho Kwon ]
• 정일윤 [ Ill Yun Jeong ]
• 변명우 [ Myung Woo Byun ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국식품과학회지 [Korean Journal of Food Science and Technology]

• 간기

  격월간

• pISSN

  0367-6293

• 수록기간

  1969~2011

• 십진분류

  KDC 574 DDC 664