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Antitumor Activities of Spray-dried Powders with Different Molecular Masses Fractionated from the Crude Protein-bound Polysaccharide Extract of Agaricus blazei Murill

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 16 Number 4 (2007.08)바로가기
  • 페이지
    pp.600-604
  • 저자
    Hong, Joo-Heon, Kim, Seok-Joong, Ravindra, Pogaku, Youn, Kwang-Sup
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79214

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원문정보

초록

영어
In this study, we first prepared 3 kinds of powders with different molecular masses from the crude protein-bound polysaccharide extract of Agraricus blazei Murill through ultrafiltration, followed by spray-drying. Then, the antitumor activities of the powders were analyzed. Size exclusion chromatography coupled with a multi-angle laser-light-scattering system showed the 3 powders had the following molecular ranges: below 10 kDa (SD-1), 10 to 150 kDa (SD-2), and above 150 kDa (SD-3), representing peak molecular weights of 8.26x103, 9.65x104 and 5.94x106 g/mol, respectively. All the powders stimulated macrophage RAW264.7 cells to produce nitric oxide, of which SD-2 and SD-3 were superior to the crude extract powder (CP-SD), while SD-1 showed the lowest activity. Similar results were found for their cytotoxicities against human cancer cell lines (A549, MCF-7, and AGS), where the highest activity was obtained with the SD-2 treatment for 72 hr at 1,000 ug/mL. The MCF-7 cell line was less sensitive to the powders than the other cells. From this research we found that ultrafiltration, in combination with spray-drying, is applicable for preparing protein-bound polysaccharide powders with higher antitumor activities.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

Agaricus blazei Murill protein-bound polysaccharide antitumor activity molecular weight distribution ultrafiltration

저자

  • Hong, Joo-Heon [ Bio Industry Center, Daegu New Technology Agency ]
  • Kim, Seok-Joong [ Department of Food and Nutrition, Dongduk Women's University ]
  • Ravindra, Pogaku [ School of Engineering and Information Technology, University Malaysia Sabah ]
  • Youn, Kwang-Sup [ Department of Food Science and Technology, Catholic University of Daegu ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 16 Number 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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