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Development and Evaluation of an Immunochromatographic Assay for Screening Listeria spp. in Pork and Milk

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 16 Number 4 (2007.08)바로가기
  • 페이지
    pp.515-519
  • 저자
    Kim, Seong-Hee, Kim, Jin-Young, Han, Woong, Jung, Byeong-Yeal, Chuong, Pham-Due, Joo, Hae-Jin, Ba, Hoa-Van, Son, Won-Geun, Jee, Young-Heun, Yoon, Byoung-Su, Lee, Yong-Soon, Lim, Yoon-Kyu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79199

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원문정보

초록

영어
Rapid immunochromatographic assay (ICA) kits were developed using flagella-specific monoclonal antibodies (MAbs) and rabbit polyclonal antibodies for screening Listeria spp. in food. The establishment of different formats, MAb 2B1 as capture antibody and MAb 7A3 or rabbit polyclonal antibodies as detector antibody, was compared. The 2 formats of the ICA kit were shown to have specific reactions with Listeria and no cross-reactivity with any of the non-Listeria including Escherichia coli O157:H7 and Salmonella enteritidis. The detection limits of the ICA kit using the combination of gold-labeled MAb 7A3 and MAb 2B1 showed 1x105 and 1x106 CFU/0.1 mL at 22 and 30℃, respectively. The other format of the ICA kit using the combination of gold-labeled rabbit polyclonal antibodies and MAb 2B1 showed 1x106 CFU/0.1 mL at 22℃ but weak signal at 30 culture. The format utilizing MAb was more sensitive than the one using polyclonal antibodies for capture antibody. Samples contaminated with L. monocytogenes 4b culture (9-10, 5-6, and 1-2 CFU/mL) on pork and pasteurized milk were confirmed as positive results. Current data suggests that this ICA kit is a rapid, simple and effective tool to screen for Listeria spp. in food.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

Listeria immunochromatographic assay food flagellum monoclonal antibody (MAb) rabbit polyclonal antibody

저자

  • Kim, Seong-Hee [ National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry ]
  • Kim, Jin-Young [ Jeju Provincial Institute for Live Stock Promotion ]
  • Han, Woong [ ViroMed Co., Ltd. ]
  • Jung, Byeong-Yeal [ National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry ]
  • Chuong, Pham-Due [ Faculty of Animal Science and Veterinary Medicine, Thainguyen University of Agriculture and Forestry ]
  • Joo, Hae-Jin [ of Veterinary Medicine, Cheju National University ]
  • Ba, Hoa-Van [ Department of Veterinary Medicine, Cheju National University ]
  • Son, Won-Geun [ Department of Veterinary Medicine, Cheju National University ]
  • Jee, Young-Heun [ Department of Veterinary Medicine, Cheju National University ]
  • Yoon, Byoung-Su [ Department of Biology, Kyonggi University ]
  • Lee, Yong-Soon [ Department of Veterinary Medicine, Seoul National University ]
  • Lim, Yoon-Kyu [ Department of Veterinary Medicine, Cheju National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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