Earticle

초록

영어

To investigate production and hydrolysis of levan, the levansucrase enzymes from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071 were used. The optimum temperature of R. aquatilis levansucrase for levan formation was 37℃, whereas that of Z. mobilis was 4℃, under the experimental conditions. Both levansucrases also catalyzed the reverse levan hydrolysis reaction. Levan hydrolysis reactions from both levansucrases were temperature dependent; high temperature (20℃) was more favorable than low temperature (4℃) by 4 times. Fructose was the only product from hydrolysis reaction by both levansucrases, showing that both levansucrases mediated the hydrolysis reaction of exo-enzyme acting. In both enzymes, initial levan hydrolysis activity was almost accounted to 1% of initial levan formation activity. The results allow the estimation of the fructose release rate in enzyme processing conditions.

목차

Abstract
 Introduction
 Materials And Methods
 Results  and Discussion
 References

키워드

저자

• Jang, Ki-Hyo [ Department of Food and Nutrition, Kangwon National University ]
• Kang, Soon-Ah [ Department of Fermented Food Science, Seoul University of Venture and Information ]
• Kim, Chul-Ho [ Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology ]
• Lee, Jae-Cheol [ Department of Food and Nutrition, Kangwon National University ]
• Kim, Mi-Hyun [ Department of Food and Nutrition, Kangwon National University ]
• Son, Eun-Wha [ Department of Pharmacognosy Material Development, Kangwon National University ]
• Rhee, Sang-Ki [ Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664