Effects of the Methanol Extract of the Leaves of Brassica juncea and Its Major Component, Isorhamnetin 3-O-B-D-Glucoside , on Hepatic Drug Metabolizing Enzymes in Bromobenzene-treated Rats
The effects of the methanol extract of the leaves of Brassica juncea and isorhamnetin 3-O-B-D-glucopyranoside, major compound isolated from the ethyl acetate fraction of this plant on hepatic lipid peroxidation and drug-metabolizing enzymes, were evaluated in rats treated with bromobenzene. The extract and isorhamnetin 3-O-B-D-glucopyranoside of oral administration did not show any significant effects on activities of aminopyrine N-demethylase and aniline hydroxylase, enzymes forming toxic epoxide by bromobenzene as well as on glutathione content. However, both methanol extract and isorhamnetin 3-O-B-D-glucopyranoside significantly recovered the decreased activities of glutathione s-transferase and epoxide hydrolase, and also reduced the lipid peroxide level in rats treated with bromobenzene. From the results, the protections of this plant against bromobenzene-induced hepatotoxicity are thought to be via enhancing the activities of epoxide hydrolase and glutathione s-transferase, enzymes removing toxic epoxide, and reducing the lipid peroxide level.
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Abstract Introduction Materials and Methods Results and Discussion Acknowledgments References