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Purification and Evaluation of Rice Bran Hydrolysates with Antimutagenicity

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 16 Number 2 (2007.04)바로가기
  • 페이지
    pp.285-289
  • 저자
    Heo, Seok, Hettiarachy, Navam, Park, Jong-Seok, Kim, Hyung-Il, Paik, Hyun-Dong, Yun, Mi-Suk, Lee, Si-Kyung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79158

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원문정보

초록

영어
A 3% suspension of heat-stabilized defatted rice bran was treated with papain, followed by inactivating the enzyme by heat, and centrifuged. The supernatant was subjected to ultrafiltration, and fractions with various molecular sizes, F1 (>30 kDa), F2 (10-30 kDa), F3 (5-10 kDa), F4 (3-5 kDa), and F5 (3 kDa<), were freeze-dried, and evaluated for antimutagenicity by Ames test using Salmonella typhimurium TA 100 against phenazine methosulfate. The F3 fraction containing highest antimutagenicity from ultrafiltration was separated into 6 fractions by DEAE-Sephadex A-25 ion-exchange column chromatography (F3-1-F3-6). Each fractions having protein contents were pooled, dialyzed, freeze dried, and evaluated for antimutagenicity. Among the six fractions, the F3-1, F3-2, and F3-6 fractions showed antimutagenicity, which were 80.2, 53.4, and 58.6% at concentration of 100 ug/plate, respectively. These F3-1, F3-2, and F3-6 fractions were subjected to Sephadex G-50 gel filtration column chromatography for further purification. Among the purified fractions, the F3-1-1, F3-2-2, and F3-6-1 fractions showed antimutagenicity of 84.5, 58.6, and 69.8% at concentration of 100 ug/plate, respectively. It is thought that these peptides can find application for nutraceutical and pharmaceutical products.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

저자

  • Heo, Seok [ Department of Applied Biology and Chemistry, Konkuk University ]
  • Hettiarachy, Navam [ Department of Food Science, University of Arkansas ]
  • Park, Jong-Seok [ Department of Food Science and Technology, Dongguk University ]
  • Kim, Hyung-Il [ Department of Food Science and Technology, Dongguk University ]
  • Paik, Hyun-Dong [ Department of Food Science and Biotechnoogy of Animal Resources, Konkuk University ]
  • Yun, Mi-Suk [ Department of Applied Biology and Chemistry, Konkuk University ]
  • Lee, Si-Kyung [ Department of Applied Biology and Chemistry, Konkuk University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 16 Number 2

    피인용수 : 0(자료제공 : 네이버학술정보)

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