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Purification and Evaluation of Rice Bran Hydrolysates with Antimutagenicity

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 16 Number 2 (2007.04)바로가기
  • 페이지
    pp.285-289
  • 저자
    Heo, Seok, Hettiarachy, Navam, Park, Jong-Seok, Kim, Hyung-Il, Paik, Hyun-Dong, Yun, Mi-Suk, Lee, Si-Kyung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79158

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원문정보

초록

영어
A 3% suspension of heat-stabilized defatted rice bran was treated with papain, followed by inactivating the enzyme by heat, and centrifuged. The supernatant was subjected to ultrafiltration, and fractions with various molecular sizes, F1 (>30 kDa), F2 (10-30 kDa), F3 (5-10 kDa), F4 (3-5 kDa), and F5 (3 kDa<), were freeze-dried, and evaluated for antimutagenicity by Ames test using Salmonella typhimurium TA 100 against phenazine methosulfate. The F3 fraction containing highest antimutagenicity from ultrafiltration was separated into 6 fractions by DEAE-Sephadex A-25 ion-exchange column chromatography (F3-1-F3-6). Each fractions having protein contents were pooled, dialyzed, freeze dried, and evaluated for antimutagenicity. Among the six fractions, the F3-1, F3-2, and F3-6 fractions showed antimutagenicity, which were 80.2, 53.4, and 58.6% at concentration of 100 ug/plate, respectively. These F3-1, F3-2, and F3-6 fractions were subjected to Sephadex G-50 gel filtration column chromatography for further purification. Among the purified fractions, the F3-1-1, F3-2-2, and F3-6-1 fractions showed antimutagenicity of 84.5, 58.6, and 69.8% at concentration of 100 ug/plate, respectively. It is thought that these peptides can find application for nutraceutical and pharmaceutical products.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

rice bran hydrolysate antimutagenic activity ultrafiltration papain

저자

  • Heo, Seok [ Department of Applied Biology and Chemistry, Konkuk University ]
  • Hettiarachy, Navam [ Department of Food Science, University of Arkansas ]
  • Park, Jong-Seok [ Department of Food Science and Technology, Dongguk University ]
  • Kim, Hyung-Il [ Department of Food Science and Technology, Dongguk University ]
  • Paik, Hyun-Dong [ Department of Food Science and Biotechnoogy of Animal Resources, Konkuk University ]
  • Yun, Mi-Suk [ Department of Applied Biology and Chemistry, Konkuk University ]
  • Lee, Si-Kyung [ Department of Applied Biology and Chemistry, Konkuk University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 16 Number 2

    피인용수 : 0(자료제공 : 네이버학술정보)

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