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The Protective Effect of Chlorophyll a Against Oxidative Stress and Inflammatory Processes in LPS-stimulated Macrophages

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 16 Number 2 (2007.04)바로가기
  • 페이지
    pp.205-211
  • 저자
    Park, Ji-Young, Park, Chung-Mu, Kim, Jin-Ju, Noh, Kyung-Hee, Cho, Chung-Won, Song, Young-Sun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79143

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원문정보

초록

영어
This study was designed to investigate the suppressive effect of chlorophyll a on nitric oxide (NO) production and intracellular oxidative stress. In addition, chlorophyll a regulation of nuclear factor (NF) xB activation and inducible NO synthase (iNOS) expression were explored as potential mechanisms of NO suppression in a lipopolysaccharide (LPS)-stimulated macrophage cell line. RAW 264.7 murine macrophages were preincubated with various concentrations (0-10 ug/mL) of chlorophyll a and stimulated with LPS to induce oxidative stress and inflammatory response. Treatment with chlorophyll a reduced the accumulation of thiobarbituric acid-reactive substances (TBARS), enhancing glutathione level and the activities of antioxidative enzymes including superoxide dismutase, catalase, glutathione peroxidase (GSH-px), and glutathione reductase in LPS-stimulated macrophages compared to LPS-only treated cells. NO production was significantly suppressed in a dose-dependent manner (p<0.05) with an IC50 of 12.8 ug/mL. Treatment with chlorophyll a suppressed the levels of iNOS protein and its mRNA expression. The specific DNA binding activities of NFkB on nuclear extracts from chlorophyll a treated cells were significantly suppressed in a dose-dependent manner with an IC50 of 10.7 ug/mL. Chlorophyll a ameliorates NO production and iNOS expression through the down-regulation of NFkB activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.

목차

Abstract
 Introduction
 Materials and Methods 
 Results and Discussion
 Acknowledgments
 References

키워드

chlorophyll a oxidative stress inflammation iNOS expression NO macrophage

저자

  • Park, Ji-Young [ BK21 Center of Smart Food and Drug, Biohealth Products Research Center, Inje University ]
  • Park, Chung-Mu [ BK21 Center of Smart Food and Drug, Biohealth Products Research Center, Inje University ]
  • Kim, Jin-Ju [ BK21 Center of Smart Food and Drug, Biohealth Products Research Center, Inje University ]
  • Noh, Kyung-Hee [ BK21 Center of Smart Food and Drug, Biohealth Products Research Center, Inje University ]
  • Cho, Chung-Won [ School of Biomedical and Biotechnology, Inje University ]
  • Song, Young-Sun [ BK21 Center of Smart Food and Drug, Biohealth Products Research Center, Inje University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 16 Number 2

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