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Effects of Oenanthe javanica on Transcriptional Regulation of COX-2 by Inhibiting Translocation of p65 Subunit in LPS-Stimulated Murine Peritoneal Macrophages

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 바로가기
  • 통권
    Volume 15 Number 6 (2006.12)바로가기
  • 페이지
    pp.975-979
  • 저자
    Lee, Jeong-Min, Kim, Hyun-Ji, Choi, Hee-Jung, You, Yang-Hee, Hwang, Kwon-Tack, Lee, Myung-Yul, Park, Chang-Soo, Jun, Woo-Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79102

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원문정보

초록

영어
The extracts of Oenanthe javanica were evaluated for their effects on the expression of cyclooxygenase-2 (COX-2), which is mediated by the translocation of the p65 subunit into the nucleus. Fractions of ethyl acetate and chloroform from 80% ethanol extracts of O. javanica exhibited inhibitory effects on the secretion of tumor necrosis factor-a (TNF-a) from lipopolysaccharide (LPS)-stimulated peritoneal macrophages; however, the aqueous- and hexane-fractions showed no significant effect. The ethyl acetate- and chloroform-fractions also reduced the COX-2 enzyme levels after 24-hr treatment. RT-PCR showed that the mRNA levels of COX-2 decreased following treatment with these fractions, suggesting that COX-2 expression is transcriptionally regulated by these extracts. We examined the effects of the chloroform- and ethyl acetate-fractions on the cytosolic activation of nuclear factor-xB (NF-xB, p65 subunit) and on the degradation of inhibitor-xBa (I-xBa) in order to determine the mechanism of COX-2 regulation. The LPS-stimulated activation of the p65 subunit was significantly blocked upon the addition of 50 ug/mL of these fractions, and the cytosolic I-xBa degradation process was simultaneously inhibited. These findings suggest that the inhibition of COX-2 expression by the ethyl acetate-and chloroform-fractions may result from the inhibition of p65 translocation by blocking the degradation of I-xBa; this may be the mechanistic basis for the anti-inflammatory effects of O. javanica.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

Oenanthe javanica;NF-xB p65 subunit COX-2;I-xB INF-a

저자

  • Lee, Jeong-Min [ Department of Food and Nutrition, Nambu University ]
  • Kim, Hyun-Ji [ Department of Food and Nutrition, Nambu University ]
  • Choi, Hee-Jung [ School of Education, Chonnam National University ]
  • You, Yang-Hee [ Department of Food and Nutrition, Chonnam National University ]
  • Hwang, Kwon-Tack [ Department of Food and Nutrition, Nambu University ]
  • Lee, Myung-Yul [ Department of Food and Nutrition, Chosun University ]
  • Park, Chang-Soo [ Department of Pathology, Chonnam National University Medical School ]
  • Jun, Woo-Jin [ Department of Food and Nutrition, Chonnam National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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