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Development of Detection Method for Cyclomaltodextrinase Family Genes using Degenerate PCR Primers

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 바로가기
  • 통권
    Volume 15 Number 6 (2006.12)바로가기
  • 페이지
    pp.967-974
  • 저자
    Oh, Su-Won, Jang, Myoung-Uoon, Jeong, Chang-Ku, Yuk, Jeong-Bin, Park, Jung-Mi, Park, Kwan-Hwa, Kim, Tae-Jip
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A79101

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원문정보

초록

영어
Cyclomaltodextrinases (CDases), maitogenic amylases, and neopullulanases share highly conserved primary structures and similar characteristics, and are thus classified into the same family. BLAST search has showed that a variety of bacterial strains harbor putative CDase family genes with several well-conserved motif amino acid sequences. In this study, four degenerate polymerase chain reaction (PCR) primer sets were designed for the detection of CDase genes, on the basis of their highly conserved amino acid blocks (WYQIFP, DGWRLD, LGSHDT, and KCMVW). The PCR detection conditions were optimized and the detection specificity of each for the primer sets was tested against the genomic DNAs isolated from 23 different Bacillus-associated species. Consequently, all tested primer sets evidenced successful amplification of specific PCR products in length, which share 55-98% amino acid sequence identity with known and putative CDases. The primers developed herein, therefore, can be applied for the easy and efficient detection and isolation of CDase family genes for the modification of functional food carbohydrates.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

degenerate PCR primers cyclomaltodextrinases maltogenic amylases neopullulanases functional food carbohydrates

저자

  • Oh, Su-Won [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ]
  • Jang, Myoung-Uoon [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ]
  • Jeong, Chang-Ku [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ]
  • Yuk, Jeong-Bin [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ]
  • Park, Jung-Mi [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ]
  • Park, Kwan-Hwa [ Center for Agricultural Biomaterials, and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University ]
  • Kim, Tae-Jip [ Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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