The chemical reduction of Pseudomonas syringae subsp. phaseolicola alginates produces neutral polymers of D-mannose and L-gulose in source specific ratios. L-Gulose was highly sensitive to degradation by 1N HCl at 100℃. As hydrolysis time increased, gulose recovery decreased to 22% after 4 hr, whereas 98% of the D-mannose was recovered under the same conditions. Thin layer chromatography showed the formation of a second product upon L-gulose acid hydrolysis. This new product had a rate of flow (Rf) value of 0.58, identical to that of 1,6 anhydro-B-D-mannopyranose and very close to that of 1,6 anhydro-B-D-glucopyranose (Rf=0.60). Because of the difference in acid sensitivity between L-gulose and D-mannose, normal acid hydrolytic techniques applied to reduced alginates produces erroneous mannuronic acid (M): guluronic acid (G) ratio's unless one accounts for the differential rates of destruction of each sugar.
목차
Abstract Introduction Materials and Methods Results and Discussion References
키워드
acid sensitivitygulosemannosealginates
저자
Ashby, Richard D. [ Audubon Sugar Institute, Louisiana State University Agricultural Experiment Station ]
Day, Donal F. [ Audubon Sugar Institute, Louisiana State University Agricultural Experiment Station ]
Kim, Du-Woon [ Department of Food and Nutrition, Chonnam National University ]
Corresponding author