Earticle

초록

영어

To determine whether the toxicity of Bacillus cereus would be seen in human cell lines and mice, we screened B. cereus B-38B, B. cereus B-50B, and B. cereus KCCM40935 for genes that coded for 5 enterotoxins using the polymerase chain reaction and cultivated them for 17 hr, by whose time they had grown to 107-108 colony-forming units (CFU) per milliliter. Cell-free supernatant was added to make up 1% of the total reaction solution. Human cells from normal lung, lung carcinoma, embryonic kidney, and cervical adenocarcinoma cell lines were grown in culture. The cytotoxicity induced by adding the reaction solution was indicated by cell death rates of 0 to 70%, depending on the bacterial strain involved and the cell line. A lethality of 20% was observed when B. cereus cultures containing 107-108 viable cells were administrated orally to mice. Therefore, the culture of B. cereus containing 107-108 viable cells seems to have high cytotoxicity on human cell lines and lethality on mice.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자

• Lee, No-A [ Department of Food Science and Biotechnology, Kyungwon University ]
• Chang, Hak-Gil [ Department of Food Science and Biotechnology, Kyungwon University ]
• Kim, Hyun-Pyo [ Pulmuone Functional Materials R&D Center ]
• Kim, Hyun-Su [ Department of Microbial Engineering, Konkuk University ]
• Park, Jong-Hyun [ Department of Food Science and Biotechnology, Kyungwon University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664