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Rapid High Performance Liquid Chromatographic Quantification of Major Isoflavones in Soybeans and Soybean Pastes

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 바로가기
  • 통권
    Volume 15 Number 1 (2006.02)바로가기
  • 페이지
    pp.24-27
  • 저자
    Kim, Won-Chan, Kwon, Soon-Ho, Rhee, In-Koo, Hur, Jong-Moon, Jeong, Hyun-Hee, Choi, Sun-Ha, Lee, Kyung-Bok, Kang, Young-Hwa, Song, Kyung-Sik
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A78931

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원문정보

초록

영어
A simple HPLC quantification method was developed for genistein, genistin, daidzein, and daidzin in soybeans and soybean products. The procedure used a 4.6x100 mm Chromolith(R) RP-18e column with a mobile phase of 1% HOAc in 20% MeOH to 1% HOAc in 80% MeOH for 10 min. The injection volume was 2 uL at a flow rate of 2 mL/min. Detection was carried out under UV at 254 nm. Under these conditions, the major isoflavones daidzein, daidzin, genistein, and genistin in soybean and soybean pastes were eluted within 7 min with baseline separation. Optimal extraction of the above four major isoflavones was achieved when 40 g of soybean or soybean paste was refluxed in 100 mL of 95% ethanol for 2 hr. Ten different soybean cultivars and nine commercial soybean pastes were analyzed by this method. The total isoflavone content was highest in the cultivar Somyung ( 2,497 ug/g dry weight). The isoflavone content in soybean pastes varied widely from manufacturer to manufacturer (an almost five-fold difference between the highest and lowest values). Such variations were presumably due to differences in fermentation conditions, type of soybeans used, and levels of such additives as starch and salt.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

high performance liquid chromatography soybean isoflavone isoflavone glycoside quantification

저자

  • Kim, Won-Chan [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Kwon, Soon-Ho [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Rhee, In-Koo [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Hur, Jong-Moon [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Jeong, Hyun-Hee [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Choi, Sun-Ha [ Department of Biochemistry, School of Medicine, Konyang University ]
  • Lee, Kyung-Bok [ Department of Biochemistry, School of Medicine, Konyang University ]
  • Kang, Young-Hwa [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ]
  • Song, Kyung-Sik [ Division of Applied Biology & Chemistry, College of Agriculture & Life Sciences, Kyungpook National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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