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Characterization and Application of a Novel Thermostable Glucoamylase Cloned from a Hyperthermophilic Archaeon Sulfolobus tokodaii

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 바로가기
  • 통권
    Volume 14 Number 6 (2005.12)바로가기
  • 페이지
    pp.860-865
  • 저자
    Njoroge, Rose Nyawira, Li, Dan, Park, Jong-Tae, Cha, Hyun-Ju, Kim, Mi-Sun, Kim, Jung-Wan, Park, Kwan-Hwa
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A78922

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원문정보

초록

영어
A gene for a putative glucoamylase, stg, of a hyperthermophilic archae on Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The recombinant glucoamylase (STGA) had an optimal temperature of 80℃ and was extremely thermostable with a D-value of 17 hr. The pH optimum of the enzyme was 4.5. Being different from fungal glucoamylases, STGA hydrolyzed maltotriose (G3) most efficiently. Gel permeation chromatography and sedimentation equilibrium analytical ultracentrifugation analysis showed that the enzyme existed as a dimer. STGA was stable enough to hydrolyze liquefied com starch to glucose in 4 hr at with a yield of95%. Comparison of the Kcal values for the hydrolysis and the reverse reaction at 75℃ and 90℃ indicated that glucose production by STGA was more efficient at 90℃ than 75℃. Therefore, STGA showed great potential for application to the industrial glucose production process due to its high thermostability.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

Sulfolobus tokodaii glucoamylase (STGA) reversion thermostability

저자

  • Njoroge, Rose Nyawira [ Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University ]
  • Li, Dan [ National R&D Center for Soybean Processing, and College of Biological Science and Technology, Changchun University ]
  • Park, Jong-Tae [ Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University ]
  • Cha, Hyun-Ju [ Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University ]
  • Kim, Mi-Sun [ Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University ]
  • Kim, Jung-Wan [ Department of Biology, University of Incheon ]
  • Park, Kwan-Hwa [ Center for Agricultural Biomaterials and School of Agricultural Biotechnology, Seoul National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 14 Number 6

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