Earticle

초록

영어

Although action modes of equol and genistein have been extensively studied, their precise roles in tumor cells remain elusive. To address possible effects of these compounds on protein expression in mammary tumor cells, proteins modulated in MCF-7 mammary tumor cells when incubated in absence and presence of 10 uM equol or genistein were identified through 2-dimensional gel electrophoresis, MALDI-TOF MS/MS, and NCBInr database search using Mascot software. Most proteins differentially expressed in MCF-7 cells after treatment with 10 uM genistein or equol were identified as being the same. Exposure to both compounds caused decreased cellular expression of RNA-binding protein regulatory subunit and oncogene DJ1 tubulin beta-1 chain, and increased expression of heterogeneous ribonucleoproteins F and L, KH-type splicing regulatory protein, and translation elongation factor EF-Tu precursor. Genistein and equol at dose used in this study showed common action mechanism.

목차

Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Acknowledgments
 References

키워드

저자

• Kim, Jang-Hoon [ Department of Animal Science and Biotechnology, Kyungpook National University ]
• Lim, Hyun-Ae [ Department of Animal Science and Biotechnology, Kyungpook National University ]
• Lee, Jeong-Soon [ Department of Animal Science and Biotechnology, Kyungpook National University ]
• Sung, Mi-Kyung [ Department of Food and Nutrition, Sookmyung Women's University ]
• Kim, Young-Kyoon [ Department of Forestry Products, Kookmin University ]
• Yu, Ri-Na [ Department of Food and Nutrition, University of Ulsan ]
• Kim, Jong-Sang [ Department of Animal Science and Biotechnology, Kyungpook National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664