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Hypocholesterolemic Soybean Peptide (IAVP) Inhibits HMG-CoA Reductase in a Competitive Manner
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- 발행기관
- 한국식품과학회 바로가기
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- 간행물
- Food Science and Biotechnology SCIE KCI 등재 바로가기
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- 통권
- Volume 14 Number 6 (2005.12)바로가기
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- 페이지
- pp.727-731
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A78897
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Synthesized Ile-Ala-Val-Pro (IAVP) peptide, which has the highest hypocholesterolemic effect among a number of synthesized derivatives of Ile-Ala-Val-Pro-Gly-Glu-Val-Ala (IAVPGEVA) isolated from 11S globulin of soy protein by pepsin digestion, was selected for investigation in the present study. Using a recombinant Syrian hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), we studied in detail the inhibition of this enzyme by IAVP and compared the action of this peptide to that of lovastatin, a known competitive inhibitor of this enzyme. The concentration of IAVP required for 50% inhibition (IC50) of HMGR activity in given experimental conditions was340 uM . Kinetic analysis revealed that the studied peptide is a competitive inhibitor of HMGR with respect to both 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) and nicotinamide adenine dinucleotide phosphate (NADPH), with an equilibrium constant of inhibitor binding (Ki = [K][I]/[KI] of 61±1.2 uM and 157±4.4 uM , respectively. At the same conditions, Ki and IC50 for lovastatin were 2.2±0.1 uM and 12.5 nM, respectively. Thus, the given peptide interacts with HMGR as a bisubstrate, consequently blocking access of both substrates to the active sites. The achieved results suggest the design of new peptide sequences having a higher relative affinity to binding sites of this enzyme and an enhancement of their hypocholesterolemic properties.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
키워드
Ile-Ala-Val-Pro peptide hypocholesterolemic peptide competitive inhibitor 3-hydroxy-3-methylglutaryl coenzyme A reductase저자
간행물 정보
발행기관
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- 발행기관명
- 한국식품과학회 [Korean Society of Food Science and Technology]
- 설립연도
- 1970
- 분야
- 농수해양>식품과학
- 소개
- 본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.
간행물
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- 간행물명
- Food Science and Biotechnology
- 간기
- 격월간
- pISSN
- 1226-7708
- 수록기간
- 1992~2009
- 십진분류
- KDC 574 DDC 664
이 권호 내 다른 논문 / Food Science and Biotechnology Volume 14 Number 6
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