Jo, Jin-Ho, Do, Jeong-Ryong, Kim, Young-Moung, Kim, Dong-Soo, Lee, Taek-Kyun, Kim, Seon-Bong, Cho, Seung-Mock, Kang, Suk-Nam, Park, Douck-Choun
언어
영어(ENG)
URL
https://www.earticle.net/Article/A78881
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원문정보
초록
영어
Enzymatic hydrolysis of shark (Squatina oculata) cartilage (SC) was optimized by response surface methodology (RSM) for chondroitin sulfate (CS) preparation. Among 11 commercial proteases, Maxazyme NNP showed highest productivity (CS yield per enzyme cost) of CS. Optimal hydrolysis conditions determined by RSM were 1.63% and 2.87 hr for enzyme concentration and hydrolysis time (r2 = 0.9527,p<0.01), respecively and highest yield of hydrolysate under the conditions was 42.3%. The yield (43.1±2.1%) and CS content (24.8±0.1%) of hydrolysate at optimal condition verified statistical optimization of SC enzymatic hydrolysis was valid.
목차
Abstract Introduction Materials and Methods Results and Discussion References