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Use of Restriction Fragment Length Polymorphism Analysis to Differentiate Fungal Strains in Sunchang Meju

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 17 Number 4 (2008.08)바로가기
  • 페이지
    pp.888-891
  • 저자
    Jong-Hyun Jung, Dong-Ho Seo, Sung-Hee Bhoo, Suk-Jin Ha, Jong-Sang Kim, Jeong Hwan Kim, Dae Young Kwon, Jaeho Cha, Cheon-Seok Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A78610

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원문정보

초록

영어
Twenty-three fungal strains were isolated from meju that had originated from the Sunchang province, the famous location for making fermented soybean foods in Korea. The restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rDNA (ITS-RFLP) was applied to differentiate the isolated fungal strains. First, the ITS region by polymerase chain reaction (PCR) with specific primers was amplified and then cleaved the products with different restriction enzymes. Cleavage of the amplified fragments with the restriction enzymes AluI, HaeIII, HhaI, and TaqI revealed extensive polymorphisms. The ITS-RFLP results highly correlated with ITS sequence analysis. All of the 23 fungal strains were classified into 5 groups by ITS-RFLP analysis. Aspergillus oryzae was the major fungal strain isolated from Sunchang meju (12 out of 23), while Aspergillus fumigatus was the next most frequently isolated strain (7 out of 23). In contrast, it was found that Fusarium asiaticum, Aspergillus sydowii, and Arthrinium sp. were the minor fungal strains in meju.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgment
 References

키워드

Aspergillus fumigotus Aspergillus oryzae meju internal transcribed spacer-restriction fragment lengthpolymorphism (ITS-RFLP)

저자

  • Jong-Hyun Jung [ Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University ]
  • Dong-Ho Seo [ Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University ]
  • Sung-Hee Bhoo [ Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University ]
  • Suk-Jin Ha [ Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University ]
  • Jong-Sang Kim [ Department of Animal Science and Biotechnology, Kyungpook National University ]
  • Jeong Hwan Kim [ Department of Food Science and Technology, Gyeongsang National University ]
  • Dae Young Kwon [ Korea Food Research Institute ]
  • Jaeho Cha [ Department of Biological Science, Pusan National University ]
  • Cheon-Seok Park [ Graduate School of Biotechnology, Institute of Life Science and Resources, KyungHee University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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