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Rapid Identification of Radical Scavenging Compounds in Blueberry Extract by HPLC Coupled to an On- line ABTS Based Assay and HPLC-ESI/MS

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 17 Number 4 (2008.08)바로가기
  • 페이지
    pp.846-849
  • 저자
    Chul Young Kim, Hee Ju Lee, Eun Ha Lee, Sang Hoon Jung, Dong-Un Lee, Suk Woo Kang, Sae Jin Hong, Byung-Hun Um
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A78600

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원문정보

초록

영어
This study employed high performance liquid chromatography (HPLC) coupled to an on-line ABTS+ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled ABTS+-RSD system. The negative peaks of the ABTS+-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-ABTS+-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgment
 References

키워드

blueberry Vaccinium corymbosum L. antioxidant free radical scavenging anthocyanin

저자

  • Chul Young Kim [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Hee Ju Lee [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Eun Ha Lee [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Sang Hoon Jung [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Dong-Un Lee [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Suk Woo Kang [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ]
  • Sae Jin Hong [ Department of Applied Plant Science, Kangnung National University ]
  • Byung-Hun Um [ Natural Product Research Center, KIST Gangneung Institute, Techno Valley ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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