Earticle

초록

영어

The proteolytic enzyme from Saccharomyces sp. B101 was purified to homogeneity by ammonium sulfate fractionation, ultrafiltration, diethyl aminoethyl (DEAE)-Sephadex A-50 ion-exchange chromatography, and Sephadex G-100 gel filtration chromatography from the culture supernatant of Saccharomyces sp. B101. The specific activity and the purification fold of the purified enzyme were 4,688.9 unit/mg and 18, respectively. The molecular weight of the purified enzyme was estimated to be 33 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimum pH and temperature for the enzyme activity were pH 8.5 and 30℃, respectively. The enzyme activity was relatively stable in the pH range of 6.5-8.5 at below 35℃. The salt-tolerance and stability for the enzyme activity were relatively stable even at NaCl concentrations of 10 and 15%. The activity of enzyme was inhibited by Ag²+ and , and Fe²+, activated by Mn²+. In addition, the enzyme activity was potently inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethyl sulfonylfluoride (PMSF). Based on these findings we concluded that the purified enzyme was a serine protease. Km and Vmax values for hammastein milk casein were 1.02 mg/mL and 278.38 unit/mL, respectively.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgment
 References

키워드

저자

• Joo-Yeon Hwang [ Department of Applied Biology and Chemistry, Konkuk University ]
• Sang-Moo Kim [ Faculty of Marine Bioscience and Technology, Kangneung National University ]
• Seok Heo [ Department of Applied Biology and Chemistry, Konkuk University ]
• Cheon-Jei Kim [ Department of Food Science and Biotechnolgy of Animal Resources, Konkuk University ]
• Chi-Ho Lee [ Department of Food Science and Biotechnolgy of Animal Resources, Konkuk University ]
• Si-Kyung Lee [ Department of Applied Biology and Chemistry, Konkuk University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664