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Micropropagation of Medicinal Woody Eleutherococcus pedunculus via Somatic Embryogenesis
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- 발행기관
- 강원대학교 산림과학연구소 바로가기
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- 간행물
- Journal of Forest and Environmental Science 바로가기
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- 통권
- 제23권 제1호 (2007.08)바로가기
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- 페이지
- pp.5-9
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A74880
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원문정보
초록
- 영어
- Zygotic embryos just after harvest of seeds were immature globular to heart stage. Maturation of zygotic embryos rapidly proceed when zygotic embryos together with small excised parts of endosperm were cultured on 1/3-strength MS solid medium with 2% sucrose, and the zygotic embryos were germinated within two months. Embryogenic callus was formed from the excised segments of germinating zygotic embryos of Eleutherococcus pedunclus on Murashige and Skoog (MS) medium with 4.5 μM 2,4-D. The embryogenic callus formation occurred at a low frequency (less than 7%) from hypocotyl segments. The embryogenic calli were maintained on the same medium as primary medium. High frequency somatic embryogenesis was obtained
after the cells were transferred to medium lacking 2,4-D. Cotyledonary embryos were germinated and converted into plantlets on medium with 20 μM GA3. Embryogenic callus and somatic embryos were produced spontaneously on the surfaces of roots and/or hypocotyls of plantlets. The frequency of embryogenic callus formation was 85% in roots and 34% in hypocotyls. Therefore maintain of cell lines performed very easily. Plantlets with developed epicotyls at more than 3 cm acclimatized at high frequency (89%). While plantlets with small epicotyls (less than 1 cm) were acclimatized at low rate (32%). The soil survived plantlets produced new sprouts after over wintering in the field.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Embryogenic callus and somatic embryo induction
Germination and plant conversion
RESULTS AND DISCUSSION
Rapid maturation of zygotic embryos
Embryogenic callus induction
Somatic embryo formation from embryogenic callus
Plantlet conversion
Spontaneous induction from regenerated plantlets
Soil transfer
LITERATURE CITED
키워드
Plant regeneration Acanthopanax cell culture저자
간행물 정보
발행기관
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- 발행기관명
- 강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
- 설립연도
- 1975
- 분야
- 농수해양>임학
- 소개
- 강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.
간행물
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- 간행물명
- Journal of Forest and Environmental Science [산림과학연구]
- 간기
- 계간
- pISSN
- 2288-9744
- eISSN
- 2288-9752
- 수록기간
- 1981~2025
- 등재여부
- KCI 등재
- 십진분류
- KDC 526 DDC 634
이 권호 내 다른 논문 / Journal of Forest and Environmental Science 제23권 제1호
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