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Study on the Reproductive Function in Transgenic Pig Harboring Human Erythropoietin (hEPO) Gene

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 2 (2008.06)바로가기
  • 페이지
    pp.117-121
  • 저자
    Hyun-Gi Lee, Hwi-Cheul Lee, Hak-Jae Chung, In-Sul Hwang, Myoung-Seob Choi, Sung-June Byun, Seunghoon Lee, Min-Ji Kim, Jae-Seok Woo, Won-Kyong Chang, Poongyeon Lee, Hoon-Taek Lee, Jin-Ki Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A74712

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초록

영어
Our previous study showed that transgenic (TG) pigs harboring human EPO (hEPO) gene have been shown to have reproductive disorders, including low pregnancy rates, irregular estrus cycle and low little size. To investigate these reasons, we assessed estrus behavior (standing response) and plasma 17B-estradiol (E2) level, which partly reflect reproductive function, during the estrus cycles after synchronization and superovulation by hormone treatments. Then, we analysed blood composition and expression of hEPO gene in TG pigs. Pigs were injected with PG600. After 10 days, pigs were fed with Regumate porcine for 6 days. Blood samples were collected from jugular vein. Analysis of blood composition and E2 level were measured by Hemavet 950 and E2 ELISA kit, respectively. And, the expression of hEPO gene in reproductive organs was quantitated by real-time RT-PCR. The percentage of estrus behavior in TG was significantly decreased. Hematocrit (HCT), hemoglobin (Hb) concentration and red blood cell (RBC) number were significantly higher in TG than wild type (WT). On the other hand, high expression of hEPO gene in TG was observed in the mammary gland as well as in the uterus. Moreover, plasma E2 level was significantly higher in TG than WT. These results suggest that nonspecific expression of hEPO gene in the other organs of TG may affect blood composition and plasma E2 level, thereby causing reproductive disorders.

목차

ABSTRACT
 INTRODUCTION
 MATERIAL AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

hEPO Transgenic pig E2 level Hematocrit ELISA

저자

  • Hyun-Gi Lee [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Animal Genetic Resurces Station, National Institute of Animal Science, RDA ]
  • Hwi-Cheul Lee [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Hak-Jae Chung [ Hanwoo Experimental Station, National Institute of Animal Science, RDA. ]
  • In-Sul Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Myoung-Seob Choi [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Sung-June Byun [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Seunghoon Lee [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Min-Ji Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Jae-Seok Woo [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Won-Kyong Chang [ Animal Biotechnology Division, National Institute of Animal Science, RDA ]
  • Poongyeon Lee [ Animal Genetic Resources Station, National Institute of Animal Science, RDA ]
  • Hoon-Taek Lee [ Department of Animal Science, Konkuk University ] Corresponding author
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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