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Molecular Cloning of Vps26a, Vps26b, Vps29, and Vps2635 and Expression Analysis of Retromer Complex in Micro Pig

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 1 (2008.03)바로가기
  • 페이지
    pp.65-70
  • 저자
    Ekyune Kim, Young-Hyun Kim, Chun-Hun Ryu, Jae-Woong Lee, Sang-Hyun Kim, Sang-Rae Lee, Myeong-Su Kim, Wanb-Jun Kim, Jeong mook Lim, Kyu-Tac Chang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A68363

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초록

영어
Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animal
  Total RNA Extraction and cDNA Cloning of Vps26a, Vps26b, Vps29, and Vps35 Genes from Micro Pigs
  Preparation of Protein Extracts
  Western Blot Analysis
  Recombinant DNA Constructs
  Yeast Two-Hybrid Assay
 RESULTS
  Isolation and Characterization of the Retromer Components Vps26a, Vps29, and Vps35 from Micro Pigs
  Retromer Complex Formations among Vps26a, Vps26b, Vps29, and Vps35
  Micro Pig Vps35 Interacts with Micro Pig Vps26a, Vps26b, and Vps29 in Yeast Two-Hybrid System
 DISCUSSION
 REFERENCES

키워드

Vps Retromer complex Micro pig

저자

  • Ekyune Kim [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Young-Hyun Kim [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Chun-Hun Ryu [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Jae-Woong Lee [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Sang-Hyun Kim [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Sang-Rae Lee [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Myeong-Su Kim [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Wanb-Jun Kim [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ]
  • Jeong mook Lim [ Department of Agricultural Biotechnology, Division of Animal Genetic Engineering, Seoul National University ]
  • Kyu-Tac Chang [ National Primate Research Center (NPRC), Korea Research Institute of Biotechnology(KRIBB) ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 32 No 1

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