Earticle

초록

영어

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the capacity to differentiate into various types of cells in the body. Hence, these cells may potentially be an indefinite source of cells for cell therapy in various degenerative diseases including neuronal disorders. For clinical applications of human ES cells, directed differentiation of these cells would be necessary. The objective of this study is to develop the culture condition for the expansion of neural precursor cells derived from human ES cells. Human ES cells were able to differentiate into neural precursor cells upon a stepwise culture condition. Neural precursor cells were propagated up to 5000-fold in cell numbers over 12-week period of culture and evaluated for their characteristics. Expressions of sox1 and pax6 transcripts were dramatically up-regulated along the differentiation stages by RT-PCR analysis. In contrast, expressions of oct4 and nanog transcripts were completely disappeared in neural precursor cells. Expressions of nestin, pax6 and sox1 were also confirmed in neural precursor cells by immunocytochemical analysis. Upon differentiation, the expanded neural precursor cells differentiated into neurons, astrocytes, and oligodendrocytes. In immunocytochemical analysis, expressions of type III β-tubulin and MAP2ab were observed. Presence of astrocytes and oligodendrocytes were also confirmed by expressions of GFAP and O4, respectively. Results of this study demonstrate the feasibility of long-term expansion of human ES cell-derived neural precursor cells in vitro, which can be a potential source of the cells for the treatment of neurodegenerative disorders.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Culture of Human ES Cells
  Neural Differentiation of Human ES Cells
  RT-PCR Analysis
  Cell Proliferation Analysis
  Immunochemical Analysis
 RESULTS
  Expansion and Characterization of Neural Precursor Cells
 DISCUSSION
 REFFRENCES

키워드

저자

• Deuk-Chae Na [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Sehee Kim [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Won-Ik Choi [ CHA Stem Cell Institute, Puchon CHA University College of Medicine, CHA Biotech Company Ltd., ]
• Hyun-Jin Hwang [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Inho Han [ Department of Neutrosurgery Bundang CHA Hospital ]
• Jae-hwan Kim [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Keun-Hong Park [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Hyung-Min Chung [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ]
• Seong-Jun Choi [ CHA Stem Cell Institute, Puchon CHA University College of Medicine ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636