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Effects of Insulin, Transferrin and Selenium(ITS) on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 4 (2007.12)바로가기
  • 페이지
    pp.261-265
  • 저자
    Yan Shi Quan, Kenji Naruse, Baek Chul Kim, Hong Rye Kim, Rong Xun Han, Su Min Choi, Chang Sik Park, Dong Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A58933

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초록

영어
Insulin, transferrin and selenium (ITS) complex is reported to improve in vitro development of oocytes and embryos. This study was carried out to investigate the effects of ITS during in vitro culture (IVC) of porcine parthenogenetic and nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. The electrically activated oocytes were cultured in Porcine Zygote Medium (PZM-3) with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 7 days. Also, the electrically activated reconstructed embryos were cultured in PZM-3 with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 6 days. Addition of ITS to culture medium did not affect development of porcine parthenogenetic embryos in vitro. To test the effect of ITS on the in vitro development of porcine NT embryos, factorial experiments were also performed for in vitro maturation (IVM) medium (TCM-199) with or without 1% ITS and culture medium (PZM-3) with or without 0.5% ITS. Addition of 0.5% ITS to culture medium increased (p<0.05) the proportion of NT blastocysts compared with non-treated group. In contrast, addition of 1% ITS to culture medium was ineffective or had a detrimental effect. Also, addition of ITS only to maturation medium increased (p<0.05) the percentage of NT blastocysts formation compared with the control group. In conclusion, addition of ITS to IVM or IVC medium could improve subsequent blastocyst development of porcine NT embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Oocytes 
  Production of Porcine Parthenogenetic Embryos
  Preparation of Fetal Fibroblasts
  Production of Porcine Nuclear Transfer embryos
  Evaluation of Developmental Ability of Parthenogenetic and Reconstructed Embryos
  Experimental Design
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

In vitro culture ITS Parthenogenetic embryo Nuclear transfer

저자

  • Yan Shi Quan [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Kenji Naruse [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Baek Chul Kim [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Hong Rye Kim [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Rong Xun Han [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Su Min Choi [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Chang Sik Park [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
  • Dong Il Jin [ Research Center for Transgenic and Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 4

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