Earticle

초록

영어

To examine the differential protein expression pattern in the 11.5 day post-coitus (dpc) and 18.5 dpc placenta of mouse, we have used the global proteomics approach by 2-D gel electrophoresis (2-DE) and MALDI-TOF-MS. The differential protein patterns of 3 placentae at the 11.5 dpc and 18.5 dpc from nature mating mice were analyzed. Proteins within isoelectric point range of 3.0~10.0, separately were analyzed in 2DE with 3 replications of each sample. A total of approximately 1,600 spots were detected in placental 2-D gel stained with Coomassie-blue. In the comparison of 11.5 dpc and 18.5 dpc placentae, a total of 108 spots were identified as differentially expressed proteins, of which 51 spots were up-regulated proteins such as alpha-fetoprotein, mKIAA0635 protein and transferrin, annexin A5, while 48 spots were down-regulated proteins such as Pre-B-cell colony-enhancing factor 1(PBEF), aldolase 1, A isoform, while 4 spots were 11.5 dpc specific proteins such as chaperonin and Acidic ribosomal phosphoprotein P0, while 3 spots were 18.5 dpc specific proteins such as aldo-keto reductase family 1, member B7 and CAST1/ERC2 splicing variant-1. Most identified proteins in this analysis appeared to be related with catabolism, cell growth, metabolism and regulation. Our results revealed composite profiles of key proteins involved in mouse placenta during pregnancy.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Placental Samples
  Extraction of Solubilized Proteins from Placenta
  2-D Gel Electrophoresis
  Staining and Image Analysis of 2-D-Gels
  Sample Preparation for MALDI-TOF Mass Spectro-metry Analysis
 RESULTS
  Analysis of Placenta Proteomes by 2-DE
  Identification of Differentially Expressed Spots 
 DISCUSSION
 REFERENCES

키워드

저자

• Rong Xun Han [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Hong Rye Kim [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Kenji Naruse [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Su Min Choi [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Baek-Chul Kim [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Chang Sik Park [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ]
• Dong Il Jin [ Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636