Earticle

현재 위치 Home

Viability of Somatic Cell Nuclear Transfer Embryos following Embryo Transfer in Korean Native Striped Cattle(Bos Namadicus Falconer, Chikso)

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 4 (2007.12)바로가기
  • 페이지
    pp.235-240
  • 저자
    Dae-jin Kwon, Joo-hee Park, Hwan-Sub Hwang, Yeon-Soo Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A58892

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

원문정보

초록

영어
This study was conducted to examine the viability of Korean native striped cattle (Bos namadicus Falconer, Chikso) clone embryos after embryo transfer. Chikso somatic cell nuclear transfer (SCNT) embryos were produced by fusion of ear skin cells derived from a female Chikso with enucleated oocytes matured in vitro for 18-24 hr. After in vitro culture of SCNT embryos for 7 to 8 days, fresh or vitrified blastocysts derived from SCNT were transferred into a uterine horn of recipient cows. Fifteen of total 43 recipients were pregnant at Day 50 and 4 recipients were maintained to term. Three IVF-derived calves and 1 clone Chikso calf were born. Pregnancy rate was higher when fresh embryos were transferred to recipients compared to vitrified embryos, but development to term was not different between both groups. The clone Chikso calf died at 5 days after birth due to the fullness of amniotic fluid in rumen and the infection of umbilical cord. The result of the present study shows that clone Chikso calf can produced from the embryo transfer of SCNT embryos, however, solution of abortion problem is necessary to improve the cloning efficiency.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Oocyte Collection and In Vitro Maturation (IVM)
  In Vitro Fertilization (IVF)
  Nuclear Transfer
  Vutrufucatuib if Embryos
  Preparation of Recipient Cows
  Embryo Transfer and Pregnancy Diagnosis
  Microsatellite Assay
  Statistical Analysis
 RESULTS
 DISCUSSION
 ACJBIWKEDGEMENT
 REFERENCES

키워드

SCNT Embryo transfer Vitrification Viability Chikso

저자

  • Dae-jin Kwon [ School of Veterinary Medicine and College of Animal Life Science, Kangwon National University ]
  • Joo-hee Park [ School of Veterinary Medicine and College of Animal Life Science, Kangwon National University ]
  • Hwan-Sub Hwang [ Gangwon Livestock Research Center ]
  • Yeon-Soo Park [ Gangwon Livestock Research Center ]
  • Choon-Keun Park [ College of Animal Life Science, Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Science, Kangwon National University ]
  • Hee-Tae Cheong [ School of Veterinary Medicine ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      페이지 저장