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The mRNA Expression and Methylation Pattern of Apoptosis-related and Imprinted Genes in Day 35 of Cloned Pig Fetuses

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 4 (2007.12)바로가기
  • 페이지
    pp.227-233
  • 저자
    Hyun-Ju Jung, Yeoung-Gyu Ko, Seongsoo Hwang, Gi-Sun Im, Mi-Rung Park, Jae-Seok Woo, Choon-Keum Park, Hwan-Hoo Seong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A58877

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원문정보

초록

영어
This study was conducted to examine the mRNA expression of apoptosis-related and imprinted genes and methylation pattern of the differentially methylated region (DMR) of H19 gene in day 35 of SCNT pig fetuses. The day 35 of natural mating (control) or cloned (clone) pig fetuses were recovered from uterus. Endometrium from dam and liver from fetus were obtained, respectively. mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. The Bcl-2 mRNA expression in clone was significantly lower than that of control (p<0.05). The mRNA expression of H19 gene in both endometrium and liver was significantly higher in clone than that of control, respectively (p<0.05). The level of IGF-2 mRNA in liver of clone was significantly lower than that of control (p<0.05), whereas the mRNA expression of IGF2-R gene in liver of clone was significantly higher than that of control (p<0.05). The DMR of H19 was lower methylation pattern in clone than that of control. These results suggest that the aberrant mRNA expression of apoptosis-related and imprinted genes and the lower DMR methylation pattern of imprinted gene may be closely related to the inadequate fetal development of cloned fetus.

목차

ABSTRACT
 IVTRODUCTION
 MATERIALS AND METHODS
  Recovery of Control or Cloned Fetuses
  Total RNA Extraction and cDNA Synthesis
  Quantitative Real-Time PCR
  Preparation of Genomic DNA
  Sodium bisulfite Genomic Sequencing
  Statistical Analysis
 RESULTS
  Recovery Data of Fetuses from Uterus
  mRNA Expression of Apoptosis-Related and Imprinted Genes
  DMR Methlation Pattern in H19 Gene
 DISCUSSION
 REFFTENCES

키워드

Cloned pig fetus Apotosis-related and imprinted genes Methylation Fetal growth

저자

  • Hyun-Ju Jung [ Animal Biotechnology Division, National Institute of Animal Science, College of Animal Life Science, Kangwon National University ] These autors contributed equally
  • Yeoung-Gyu Ko [ Animal Biotechnology Division, National Institute of Animal Science ] These autors contributed equally
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science ]
  • Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science ]
  • Mi-Rung Park [ Animal Biotechnology Division, National Institute of Animal Science ]
  • Jae-Seok Woo [ Animal Biotechnology Division, National Institute of Animal Science ]
  • Choon-Keum Park [ College of Animal Life Science, Kangwon National University ]
  • Hwan-Hoo Seong [ Animal Biotechnology Division, National Institute of Animal Science, ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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