Earticle

초록

영어

In this study, we investigated whether gut microbiota-derived factors contribute to the increase in intestinal permeability and the activation of left ventricular autophagy-related signaling induced by high-intensity treadmill exercise, using an antibiotic-treated rat model. Serum levels of lipopolysaccharide (LPS), LPS-binding protein (LBP), colonic occlu-din, zonulin, and myocardial autophagy-related signaling were ana-lyzed. A total of 38 specific pathogen-free male Sprague–Dawley rats aged 8 weeks were randomly assigned to four groups: control (n=9), antibiotic control (n=9), exercise (n=10), and antibiotic plus exercise (n=10). Treadmill exercise was performed for 60 min per session, 4 times per week, for 8 weeks. Eight weeks of high-intensity treadmill exercise increased intestinal permeability and elevated circulating lev-els of gut-derived LPS and LBP. Increased LPS and LBP levels were as-sociated with the activation of adenosine monophosphate-activated protein kinase (AMPK) and upregulation of myocardial autophagy-re-lated markers, including microtubule-associated protein light chain 3 and B-cell lymphoma-2-interacting myosin-like coiled-coil protein. Al-though increased intestinal permeability was also observed in the anti-biotic-treated exercise group, circulating LPS and LBP levels did not in-crease significantly, nor did AMPK activation or the expression of auto-phagy-related markers. Prolonged high-intensity treadmill exercise may be associated with increased intestinal permeability and activation of myocardial autophagy-related signaling. The attenuation of the increas-es in circulating LPS/LBP levels and in myocardial autophagy-related signaling after antibiotic treatment suggests that gut microbiota-derived factors may mediate these gut-heart responses. Optimizing exercise benefits while minimizing risks may require considering both training in-tensity and gut health in the exercise program design.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Study animals
Exercise and supplementation methods
Enzyme-linked immunosorbent assay for determine LPS and LBP serum levels
Western blot
Statistical analyses
RESULTS
Effects of high-intensity exercise on intestinal permeability markers
Effects of high-intensity exercise on serum LPS and LBP levels
Effects of high-intensity exercise on left ventricular autophagy
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Siyu Jin [ Department of Physical Education, Kunsan National University, Gunsan, Korea/Department of Sports Human Science, Hebei Sport University, Shijiazhuang, China ]
• Young-Je Sim [ Department of Physical Education, Kunsan National University, Gunsan, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613