Earticle

초록

영어

Background: Sperm cooling is a critical step in cryopreservation, as exposure to temperatures below 15℃ can induce cold shock-associated damage, including phospholipid scrambling and lipid peroxidation. Goat sperm are particularly vulnerable to cooling-induced injury because of their membrane characteristics. This study evaluated the effects of cooling rate and interval agitation during cooling in frozenthawed goat sperm. Methods: The samples were cooled at 0.5℃/min (0.5 Cool) or 0.1℃/min (0.1 Cool) and agitated every 15 min during cooling until 5℃, then, the samples were cryopreserved in liquid nitrogen. After thawing, sperm motility was assessed using computer-assisted sperm analysis (CASA) and plasma membrane integrity, acrosomal integrity, intracellular H2O2 levels, mitochondrial activity, mitochondrial membrane integrity, and mitochondrial membrane potential (ΔΨm) were analyzed by flow cytometry. Results: In results, there were no significant differences in sperm motility, plasma membrane integrity, acrosomal integrity, and mitochondrial function among the treatment groups. However, intracellular H2O2 levels were significantly lower in agitation-treated groups than non-agitated groups both 0.5 Cool and 0.1 Cool treatment groups (p < 0.05), whereas there were no significant differences between cooling rate groups. Conclusions: In conclusion, these results indicate that interval agitation during the cooling process could reduce intracellular reactive oxygen species in frozen-thawed goat sperm.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals and collection
Sperm preparation
Sperm cooling methods
Motility
Structural integrity
Intracellular H2O2 levels
Mitochondrial function
Statistical analysis
RESULTS
Effects of cooling rate and interval agitation on sperm motility
Effects of cooling rate and interval agitation on sperm structural integrity
Effects of cooling rate and interval agitation on intracellular H2O2 levels
Effects of cooling rate and interval agitation on sperm mitochondrial function
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Yeoreum Yoon [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Eunju Seok [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Minwoo Sin [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Chanjin Woo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Nahyeon Kang [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
• Eun-Do Lee [ Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea ]
• Seungjun Lee [ College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea ]
• Jongki Cho [ College of Veterinary Medicine, Seoul National University/ Farm Animal Clinical Training and Research Center, Institutes of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea ]
• Kwan-Woo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea ] Corresponding Author
• Sang-Hee Lee [ College of Animal Life Sciences, Kangwon National University/ School of ICT, University of Tasmania, Hobart 7005, Australia ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636