Earticle

초록

영어

Background: Canine mammary tumors are one of the most common tumors in female dogs, and the development of effective therapeutic agents is a critical clinical challenge. Chrysin, a natural flavonoid compound, has been reported to exhibit anticancer activity in various human cancers through the regulation of cell proliferation and apoptosis. However, the anticancer effects of chrysin on canine mammary tumors have not yet been fully elucidated. Therefore, this study investigated the antiproliferative and apoptosis-inducing effects of chrysin on CMT-U27 cells derived from canine mammary tumors. Methods: Antiproliferative activity was confirmed by treating CMT-U27 cells with chrysin at concentrations of 0-10 μM and evaluating cell viability. Cell proliferation capacity was assessed using Ki-67 immunocytochemistry. The expression levels of cell cycle-related proteins, including CDK2, CDK4, cyclin D, and cyclin E, were analyzed by Western blotting. Additionally, apoptosis-related proteins, including phosphorylated p53 and BCL2, were evaluated to investigate the apoptotic activity of chrysin treatment. Results: Chrysin treatment significantly reduced the viability and migration of CMT-U27 cells and decreased the number of Ki-67-positive cells in a dose-dependent manner. Following chrysin treatment, the expression of CDK2, CDK4, cyclin D, and cyclin E was significantly reduced, indicating that cell cycle progression was inhibited. Additionally, chrysin increased the expression of phosphorylated p53 and decreased BCL2 expression. Conclusions: Chrysin exhibited significant anticancer effects in canine mammary tumor-derived CMT-U27 cells through the inhibition of cell proliferation, accompanied by G2/M phase accumulation and downregulation of cyclin-CDK complex components. In addition, chrysin altered apoptosis-related signaling molecules, including p53 and BCL2. These results suggest that chrysin may have potential as a therapeutic candidate for canine mammary tumors.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Cell culture and chrysin treatment
Cell viability assay
Immunostaining
Flow cytometry
Cell-migration assay
Western blotting
Statistical analysis
RESULTS
Chrysin suppresses cell viability and proliferation in CMT-U27 cells
Chrysin induces cell cycle arrest in CMT-U27 cells
Chrysin induces apoptosis and inhibits migration in CMT-U27 cells
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Hyeon Woo Sim [ Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Kwanghyeon Cho [ Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea ]
• Ran Lee [ Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea ]
• Jin-Yeong Kim [ Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 27478, Korea ]
• Eun-Jung Kim [ Department of Biomedical Laboratory Science, College of Health Sciences, Sangji University, Wonju 26339, Korea ]
• Won-Young Lee [ Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea ] Corresponding Author
• Hyun-Jung Park [ Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 27478, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636