Earticle

초록

영어

Bacterial survival depends upon a sophisticated sensory repertoire capable of detecting chemical gradients, thermal fluctuations, and osmotic pressure. However, the extent to which prokaryotes can distinguish and respond to complex acoustic architectures remains a subject of skepticism and limited mechanistic understanding. Here, we report that Escherichia coli exhibits a distinct, stimulus-specific physiological response to low-frequency, rhythmically layered acoustic patterns ("Chanyang” stimulation). Macroscopic analysis revealed that while high-frequency, transient-heavy stimulation ("Rhythmic Complex") elicited a negligible phenotype, the Afro-Anthemic (Chanyang) condition triggered a transition to a hyper-proliferative, biofilm-like state. Unbiased, AI-assisted metabolomic profiling identified a global metabolic phase shift driving this phenomenon, characterized by the unique accumulation of eukaryotic-like signaling lipids, specifically N-acylglycines and N-acylethanolamines—and membrane-structuring ceramides. These findings suggest that specific acoustic structures act as abiotic stressors that are transduced into chemical signals, effectively hijacking quorum-sensing pathways to coordinate collective stress responses. We propose that the microbial "unseen logic" extends beyond chemical chemoreception to include a refined capacity for mechanotransduction of complex environmental oscillations.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Culture Conditions
2.2. Acoustic Stimulation Protocols
2.3. Metabolite Extraction and Sample Preparation
2.4. LC-MS/MS Profiling and Data Analysis
3. Results
3.1. Acoustic stimulation induces distinct morphological phenotypes
3.2. Heatmap analysis reveals global metabolic reprogramming
3.3. Molecular networking unveils unique lipid families
3.4. Enrichment of putative &quat;Endocannabinoid-like&quat; signaling lipids
3.5. Upregulation of respiratory quinones and structural lipids
4. Discussion
5. Conclusions
References

키워드

저자

• Ryoichi Yamada [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea ]
• Hirotake Yamaguchi [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea ]
• Tae-Jin Oh [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea/ Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, Asan 31460, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  선문효정학술연구회 학술대회 프로시딩

• 간기

  반년간

• 수록기간

  2023~2026

• 십진분류

  KDC 238 DDC 289