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Biochemical characteristics and molecular docking of a GH18 chitinase from lichen associated Bacillus sp. NP_108

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  • 발행기관
    선문효정학술연구회 바로가기
  • 간행물
    선문효정학술연구회 학술대회 프로시딩 바로가기
  • 통권
    Proceedings of THE 6th INTERNATIONAL CONFERENCE OF HYOJEONG ACADEMY (2025.08)바로가기
  • 페이지
    pp.96-102
  • 저자
    Lakshan Paudel, So-Ra Han, Tae-Jin Oh
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A489374

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원문정보

초록

영어
The gene encoding GH18 protein (WP_061577947.1) from lichen associated Bacillus sp.NP_108 was annotated from the CAZyme databases. Based on phylogenetic analysis and multiple sequence alignment, WP_061577947.1 was found to be the GH18 protein family. The gene (WP_061577947.1) was successfully cloned, overexpressed in Escherichia coli BL21 cells. WP_061577947.1 showed strong activity towards colloidal chitin and activity on β-1,4 galactose containing monomers however, there was no activity towards crystalline chitin and chitosan. Biochemical characterization revealed that the protein exhibited optimal activity at 50oC and a pH of 6.0. Molecular docking also highlighted the protein structure and key residue present in GH18 protein family. The catalytic activity of enzyme exhibited substrate assisted catalysis. These findings support the chitin degradation capability of Bacillus sp. NP_108 and highlight the biotechnological importance of GH18 enzyme towards production of chitooligomers.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Chemical and substrates
2.2. Cloning and purification of recombinant chitinase
2.2. Cloning and purification of recombinant chitinase
2.3. Colloidal chitin preparation and enzyme assay
2.4. Biochemical characterization
2.5. Homology modeling and molecular docking of GH18
3. Results
4. Conclusion
References

키워드

GH18 chitin hydrolase Bacillus sp. chitooligomers colloidal chitin

저자

  • Lakshan Paudel [ Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, Asan 31460, Korea ]
  • So-Ra Han [ Genome-based BioIT Convergence Institute, Asan 31460, Korea/ Bio Big Data-based Chungnam Smart Clean Research Leader Training Program, Sun Moon University ]
  • Tae-Jin Oh [ Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University/ Genome-based BioIT Convergence Institute, Asan 31460, Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    선문효정학술연구회 [Sun Moon Hyojeong Academy Society]
  • 설립연도
    2023
  • 분야
    복합학>학제간연구
  • 소개
    Journal of Hyojeong Academia aims to serve as a global platform where researchers and scholars of various disciplines can contribute ideas for our sustainable global community of Co‐existence, Co‐prosperity, and Co‐righteousness. The journal is a multidisciplinary, open‐access, internationally peer‐reviewed academic journal, and it invites all areas of research conducted in the spirit of post materialism including studies centering on God, studies unifying religions and sciences, and studies on all aspects of Co‐existence, Co‐prosperity, and Co‐righteousness.

간행물

  • 간행물명
    선문효정학술연구회 학술대회 프로시딩
  • 간기
    반년간
  • 수록기간
    2023~2026
  • 십진분류
    KDC 238 DDC 289

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