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Eco-friendly Biodegradation of PHB (Polyhydroxybutyrate) Using Newly Isolated Depolymerase-Producing Bacteria
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- 통권
- Proceedings of THE 6th INTERNATIONAL CONFERENCE OF HYOJEONG ACADEMY (2025.08)바로가기
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- 페이지
- pp.90-95
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A489373
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원문정보
초록
- 영어
- This study focuses on isolating and characterizing novel polyhydroxybutyrate (PHB) depolymerase-producing bacteria for the efficient biodegradation of PHB. PHB, a promising biodegradable alternative to petroleum-based plastics, requires specific microbial enzymes for complete degradation. We identified Janibacter terrae from contaminated soil, demonstrating high PHB degradation efficiency via clear zone formation and enzymatic activity. Scanning electron microscopy (SEM) revealed increased surface roughness and porosity in degraded PHB films, while Fourier-transform infrared spectroscopy (FTIR) confirmed the cleavage of ester bonds, indicating enzymatic breakdown. The PHB depolymerase gene was successfully cloned and expressed in E. coli, yielding a functional 58 kDa enzyme. Optimal degradation conditions were 40°C, pH 8.0, and 96 h incubation, with 0.15% PHB concentration. Glucose and yeast extract enhanced enzyme activity, whereas heavy metals like Zn²⁺ and Co²⁺ slightly stimulated depolymerase production. These findings highlight Janibacter terrae as a potent PHB degrader, offering potential for sustainable plastic waste management. Further research on degradation metabolites and enzyme engineering could enhance industrial applications
목차
Abstract
1. Introduction
2. Materials and Methods
2.1. Preparation of Emulsified PHB Medium, Films, and Degradation Assay
2.2. Scanning electron microscopy (SEM)
2.3. Fourier transform infrared (FTIR) analysis of PHB
2.4. Cloning and overexpression of PHB depolymerase
2.5. Biochemical characterization of PHB depolymerase enzyme assay
3. Results
3.1. Physical properties of PHB after biodegradation
3.2. Optimization of PHB depolymerase
4. Conclusion
5. Conclusions
References
저자
간행물 정보
발행기관
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- 발행기관명
- 선문효정학술연구회 [Sun Moon Hyojeong Academy Society]
- 설립연도
- 2023
- 분야
- 복합학>학제간연구
- 소개
- Journal of Hyojeong Academia aims to serve as a global platform where researchers and scholars of various disciplines can contribute ideas for our sustainable global community of Co‐existence, Co‐prosperity, and Co‐righteousness. The journal is a multidisciplinary, open‐access, internationally peer‐reviewed academic journal, and it invites all areas of research conducted in the spirit of post materialism including studies centering on God, studies unifying religions and sciences, and studies on all aspects of Co‐existence, Co‐prosperity, and Co‐righteousness.
간행물
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- 간행물명
- 선문효정학술연구회 학술대회 프로시딩
- 간기
- 반년간
- 수록기간
- 2023~2026
- 십진분류
- KDC 238 DDC 289
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