Earticle

초록

영어

Over the course of history, plant materials have been utilized for healing various ailments. The knowledge derived from traditional medicine has proven invaluable, serving as a foundational resource for drug discovery and design. However, the journey from screening natural products to the development of drugs is a prolonged and demanding process. Fortunately, with the emergence of sophisticated analytical techniques and the establishment of global databases containing information on thousands of natural products, the screening and discovery of bioactive compounds from complex sample mixtures have become more time-efficient and less labor-intensive. In particular, the non-targeted metabolomics approach proves advantageous, offering a comprehensive analysis of a wide range of compounds, aiding in the detection of both known and unknown substances. This approach is especially beneficial for gaining a thorough understanding of the pattern of metabolites found in Arctic and Antarctic vegetation, among the least explored areas on Earth. Here, we employed a non-targeted metabolic profiling approach to analyze the chemical components in Antarctic mosses. While many of the compounds could not be predicted, some were identified as therapeutic agents, with a few exhibiting pharmacological properties such as anticancer, anti-inflammatory, antilipemic, anti-diabetic, anti-adepogenic, and neuroprotective effects. Notably, we predicted that one moss sample produced Desulfiram, an FDA-approved drug for the treatment of alcohol dependency, and methyl palmitate, well-known for its anti-inflammatory potency, could be detected in many of the samples. Furthermore, we experimentally verified the therapeutic potential of Antarctic moss extracts by conducting a cyclooxygenase-2 (COX-2) inhibition assay, assessing their potent anti-inflammatory activity. Indeed, two of the moss samples exhibited significant COX-2 inhibitory potency. Hence, our findings emphasize the advantage of metabolic profiling in providing insight into the potential of Antarctic moss as a valuable natural resource for the discovery and development of potent therapeutics. Additional research is due in order to identify and define the particular bioactive compounds accountable for the observed effects.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Moss sample processing and extraction
2.3. Metabolic profiling
2.4. Raw data processing and molecular network building
2.5. COX-2 inhibition asssay
2.6. Statistical analysis
3. Results
3.1. Preliminary analysis of metabolites
3.2. Untargeted metabolic profiling
3.3. COX-2 inhibition activity
4. Discussion
5. Conclusions
References

키워드

저자

• Kristina Lama [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea ]
• Ryoichi Yamada [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea ]
• Hirotake Yamaguchi [ Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Korea ]
• Tae-Jin Oh [ Department of Life Science and Biochemical Engineering, Sun Moon University/ Genome-based BioIT Convergence Institute, Sun Moon University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  선문효정학술연구회 학술대회 프로시딩

• 간기

  반년간

• 수록기간

  2023~2026

• 십진분류

  KDC 238 DDC 289