Earticle

초록

영어

The variation occurs at DNA and protein level in Euryale ferox due to the exposure of gamma irradiation was investigated in the present study. Variations which are generated by treatment of 50 and 100 Gy gamma irradiations were analysed by RAPD molecular marker and SDS-PAGE molecular techniques. In case of RAPD molecular study, total 41 Polymorphic bands observed using RAPD amplification using 13 primers with an average of 3 amplicon per primer. These polymorphic amplicons could be very useful for documentation of gamma irradiated variant plants. The phylogenetic tree based on UPGMA clustered into two main clusters. SDS-PAGE analysis of protein in irradiated plants AND the control produced band patterns varied from 18 kDa to 116 kDa. The 66 kDa and 45 kDa band was produced by gamma irradiated plants. The present syudy suggest that gamma irradiation could be applied as prominent mutagenic agents in E. ferox. RAPD molecular marker and protein profile as powerful techniques allows isolation of designated mutants in breeding of E. ferox for its genetic enhancement.

목차

Abstract
Introduction
Materials and Methods
Experimental plant materials
Molecular studies of gamma irradiated Euryale ferox Salisbury samples
Genomic DNA isolation from leaf samples irradiated with gamma radiation
RAPD analysis
Statistical data analysis
Protein extraction and purification from the leaf sample of irradiated plant of Euryale ferox Salisbury
SDS-PAGE analysis
Results
DNA isolation
RAPD analysis
SDS-PAGE analysis
Discussion
Acknowledgements
References

키워드

저자

• Sanjeev Kumar [ School of Agricultural Science, KK University, Bihar Sharif 803115, India ]
• Prashant [ Department of Environmental Science, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya 824236, India ]
• Shweta Rani [ Department of Biotechnology, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya 824236, India ]
• Satyendra Gautam [ Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400085, India ]
• Vikram Singh Gaur [ College of Agriculture, Balaghat, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur 482004, India ]
• Nitish Kumar [ Department of Biotechnology, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya 824236, India ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634