Fatty Acid Profiling and Antioxidant Potential of Two-Spotted Cricket (Gryllus bimaculatus) Oil as a Sustainable Multifunctional Cosmetic Emollient
지속가능한 다기능성 화장품 에몰리언트로서 쌍별귀뚜라미(Gryllus bimaculatus ) 오일의 지방산 조성 및 항산화 특성 연구
Seung-Won Yi, So-Young Lee, Joon Ha Lee, Ji Hae Lee, Heui-Sam Lee, You-Young Jo
언어
영어(ENG)
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https://www.earticle.net/Article/A486837
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Purpose: Recent research has recognized edible insects as sustainable, high-performance bioresources for advanced functional cosmetic applications. The present study aimed to analyze the antioxidant and anti-inflammatory activities of two-spotted cricket (Gryllus bimaculatus) oil (GBO) to support resource-circulating economic practices and evaluate its potential as a sustainable, eco-friendly cosmetic emollient. Methods: The crickets were starved, washed, dried, and then their oil was extracted with a screw press at 120°C under atmospheric pressure. The fatty acid composition of GBO was analyzed using gas chromatography/mass spectrometry. The antioxidant activity was evaluated by measuring total polyphenol content (TPC), total flavonoid content (TFC), and DPPH and ABTS radical scavenging activities. Anti-inflammatory activity was assessed by examining the inhibitory effect on NO production and proinflammatory cytokine levels in RAW 264.7 cells at various concentrations of GBO. Results: The extracted GBO showed a yield of 9.7% and a rich profile of unsaturated fatty acids. The six major fatty acids identified in GBO were oleic (30.8%), palmitic (27.7%), linoleic (26.8%), stearic (8.7%), palmitoleic (2.1%), and myristic (1.02%) acids. GBO attenuated NO production and proinflammatory cytokine levels in a concentration-dependent manner and exhibited high TPCs and robust DPPH scavenging. Conclusion: As a multifunctional cosmetic ingredient that provides antioxidant efficacy, formulation-stabilizing benefits, and physiological protection, GBO represents a promising bioresource for advanced cosmeceutical applications.
한국어
목적: 식용 곤충은 우수한 생리활성을 지닌 지속가능한 바이오 자원으로 주목받고 있다. 본 연구는 자원 업사이클링 및 친환경적 화장품 소재 발굴을 위해 쌍별귀뚜라미G(ryllus bimaculatus) 유래 오일(GBO)의 항산화 및 항염증 활성을 분석하고, 지속 가능하며 친환경적인 화장품 에몰리언트 소재로서의 활용 가능성을 평가하고자 하였다. 방법: 쌍별귀뚜라미를 절식, 세척, 건조한 후, 대기압조건 및 120℃에서 스크류 프레스를 이용하여 오일을 압착 추출하였다. 쌍별귀뚜라미 오일의 지방산 조성은 GC/MS를 이용하여 분석하였고, 항산화 활성은 총 폴리페놀 함량(TPC), 총 플라보노이드 함량(TFC), DPPH 및 ABTS 라디칼 소거 활성을 측정하여 평가하였다. 결과: 세포생존율 및 항염증 활성은 다양한 농도의 쌍별귀뚜라미 오일을 처리한 RAW 264.7 세포에서 세포 생존율과 산화질소(NO) 생성 및 전염증성 세포전달물질 억제 능력을 통해 검증하였다. 쌍별귀뚜라미 오일은 9.7%의 수율을 보였으며, 풍부한 불포화 지방산 조성을 나타냈다. 쌍별귀뚜라미 오일의 6대 주요 지방산은 올레산(30.8%), 팔미트산(27.7%), 리놀레산(26.8%), 스테아르산(8.7%), 팔미톨레산(2.1%), 미리스틱산(1.02%)이었다. 쌍별귀뚜라미 오일은 농도 의존적인 방식으로 NO 생성과 전염증성 세포전달물질을 유의미하게 감소시켰으며, 특히 높은 총 폴리페놀 함량과 함께 우수한 DPPH 라디칼 소거 활성을 나타내 높은 항산화력을 나타냈다. 결론: 쌍별귀뚜라미 오일은 항산화 효능, 제형 안정화 효과 및 피부 생리학적 보호 기능을 제공하는 다기능성 화장품성분으로서, 고기능성 코스메슈티컬 제재 적용을 위한 유망 바이오 소재가 될 수 있음을 시사한다.
Abstract Introduction Materials and Methods 1. Oil Extraction from Two-spotted Crickets 2. Fatty Acid Composition 3. Determination of Total Phenolic and Flavonoid Contents 4. Determination of ABTS Radical Scavenging Activity 5. Determination of DPPH Radical Scavenging Activity 6. RAW 264.7 Cell Culture 7. Determination of Cell Viability 8. Determination of Nitric Oxide Production 9. Measurement of Level of Proinflammatory Cytokines 10. Statistical Analysis Results and Discussion 1. Physicochemical Properties of GBO 2. Fatty Acid Profile of GBO 3. Antioxidant Properties of GBO 4. Cell Viability and Cytotoxicity of GBO 5. Anti-inflammatory Effects of GBO Conclusion References 국문초록 中文摘要
Seung-Won Yi [ 이승원 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
So-Young Lee [ 이소영 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
Joon Ha Lee [ 이준하 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
Ji Hae Lee [ 이지혜 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
Heui-Sam Lee [ 이희삼 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
You-Young Jo [ 조유영 | Industrial Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, Korea ]
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