Earticle

초록

영어

The purpose of this study was to investigate the combined effects of treadmill exercise and naringenin treatment on neuronal survival and corticospinal tract (CST) fibers sprouting following spinal cord injury (SCI). Forty male rats were randomly assigned to four groups (n=10 in each group): the normal group (Norm), the SCI+sedentary group (SCI+S), the SCI+naringenin group (SCI+Na), and the SCI+naringenin combined with exercise group (SCI+Na+Ex, n=10). All rats in the SCI were allowed to recover for 2 weeks after injury, and the exercise group subsequently underwent 4 weeks of low-intensity treadmill exercise. Histological and molecular analyses were performed to evaluate astrocyte activation in the dorsal region, neuronal survival in the ventral horn, and axonal elon-gation in the caudal CST fibers following SCI. As a result, in the dorsal region of the injury site, both treatment groups showed significantly in-creased glial fibrillary acidic protein expression compared to the SCI+S group, indicating enhanced astrocyte activation. In particular, the SCI+Na+Ex group demonstrated prominent astrocytic infiltration into the cavity. In the ventral horn of the injured spinal cord, the number of mo-tor neurons was significantly preserved in the SCI+Na+Ex group com-pared to the SCI+S group. In the caudal CST, the SCI+Na+Ex group ex-hibited significantly increased neurofilament-200 expression, suggest-ing enhanced axonal regeneration. Furthermore, regeneration-associ-ated proteins including growth-associated protein-43 and activating transcription factor-3 were significantly increased following the com-bined intervention. Combined treatment of treadmill exercise and narin-genin might be one of the rehabilitation strategies to promote neural re-covery after SCI.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
SCI and naringenin extract administration
Low-intensity treadmill exercise program
Immunofluorescence staining
Western blot analysis
Statistical analysis
RESULTS
Region-specific expressions of GFAP, NF-200, and NeuN after SCI
Change in cavity through astrocyte activation after SCI
Motor neuron survival in the ventral region after SCI
Sprouting of CST fibers through reducing of the PI3K/AKT/mTOR signaling pathway after SCI
Changes in regenerative and inflammatory proteins after SCI
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Yeong-Jik Kim [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613